Immunohistochemistry guide and troubleshooting resource
New Resource: Immunohistochemistry Methods and Troubleshooting Guide
Immunohistochemistry is a laboratory technique utilized for the visual detection of antigens in tissue. When working with cells this technique is generally referred to as immunocytochemistry. These techniques are widely used by histology/pathology laboratories and life science researchers as a method to detect abnormal cells or to characterize the localization of proteins within a biological sample. Visual detection is achieved using antibodies that have been chemically conjugated with a marker such as a fluorescent dye, enzyme, radioactive element or colloidal gold. The final antibody-antigen reaction is visible using a light or fluorescent microscope depending on the labeled antibodies used for detection.
This IHC Guide covers antibody selection, methods for sample fixation and emnedding, antigen retrieval and immunostaining as well as a troubleshooting section.