New Resource: Flow Cytometry Guide and Troubleshooting

Flow cytometry is a laboratory technique used to quantify cellular or particle properties based on the scattered incident laser light and the fluorescent emissions from a beam of light projected on a focused stream of fluid.  Particles that cause light to scatter range in size from a few microns to several hundreds of microns in diameter and are traveling as a stream of single particles at a rate of thousands per second.  This technique is commonly used by life science research laboratories measuring physical and chemical characteristics of cells, as well as by diagnostic laboratories measuring patient blood/tissue samples for health related disorders.  To analyze cellular/particle properties a number of detectors along with an aligned optics system of filters and lenses are used to collect scattered light from the point where the laser beam strikes the stream of sample fluid.  Detectors are then able to record the intensity of the light and transmit this information to a computer for further analyses using flow cytometry software.  This information can ultimately provide insight on cellular populations, size, inner-structure, or be used for sorting cells.

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