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ST2 Antibodies in Different Studies and Applications

Sometimes it’s just cool to see and read about the antibodies we offer being used by customers who have published their work. Listed below are bullet points that summarize how the ST2 antibodies are being used in different studies and applications. These were written after reading customers published articles that cite the ST2 related products.  This list may relate to your work and help you optimize your experiment for one of the applications below.

Rat Anti-Mouse T1/ST2 Monoclonal Antibodies:

Immunohistochemistry:

• Detect the expression of ST2 in conjunctival tissue isolated from mice eyes. Used frozen tissue sections and examined expression patterns using confocal microscopy.
• Detect the presence of Th2 cells in four-micrometer cross sections of the eye. Eyes were obtained from C57/BL/6 and BALB/c mice.

Flow Cytometry:

• Expression of ST2 in bone marrow neutrophils isolated from BALB/c mice.
• Expression of T1/ST2 in cells isolated from lymph nodes of BALB/c mice.
• Expression of T1/ST2 in lymph node cells isolated from mice.
• Surface expression of ST2 in dendritic cells of mice.
• Expression of ST2L in splenocytes isolated from mice and in murine thymoma EL-4 cells.
• Expression of T1/ST2 on cells isolated from spleens of transgenic and wild-type mice.
• Determine the percentage of Th2 cells in the lungs of mice.
• Expression of ST2 in peritoneal cells isolated from mice.
• Expression of T1/ST2 in bone marrow cells isolated from femurs and tibia of B6129PF1/J female mice.
• Surface expression of T1/ST2 on mast cell progenitors (MCP). MCPs were isolated from the bone marrow of mice.

ELISA:• Constructed an ELISA system for determining the concentration of ST2 in mouse plasma samples, bronchoalveolar fluid and lung homogenates.
Cellular Assay:

• Blocking agent for IL-33 and IL-33R pathway analysis.

Mouse Anti-Human ST2L Monoclonal Antibodies:

Flow Cytometry:

• Expression of ST2 in cultured or freshly isolated T cells from drained lymph nodes (DLN) of BALB/c wild type mice and from CD4+ T cells of D0.11.10 mice.
• Expression profile of ST2L in CD14high T cells from human whole blood collections.
• Examine CD4+ skin resident T cells for Th1/Th2.  T cells were isolated from normal adult human skin obtained from cutaneous surgeries.