Aggrecan Antibody, N-terminal neoepitope ARG
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Mouse monoclonal Antibody to Aggrecan N-terminal neoepitope ARG. Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
Overview
During differentiation of neural precursor cells, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.
Data/Specifications
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Immunogen: ARGSV synthetic peptide conjugate. Clone: BC-3 Host: Mouse Myeloma: x63-Ag8.653 Isotype: IgG1 Light Chain type: kappa Specificity: Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognize the sequence ARG if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognize the aggrecanase generated neoepitope ARG. Cross-reactivity: human, rat, cat, cow, dog, guinea pig, horse, pig, rabbit and sheep. Purity: Affinity purified on protein G Form: Liquid, 1 mL/vial Concentration: 0.1 mg/mL Storage: -20° C |
Literature/Support
Aggrecan antibody, clone BC-3 Insert (PDF, 335 KB)
How To Use
Applications
- Western Blot - Suggested dilution: 1:100. Detects a variety of epitopes between 50 and 250 kDa
- ELISA
- IHC with frozen sections or formalin/PFA-fixed paraffin embedded sections
Technical Notes
Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units
Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG of nondeglycosylated
aggrecan for optimal epitope recognition.