ST2L Monoclonal Antibody, FITC
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Human ST2L FITC conjugated monoclonal antibody (clone B4E6) for the identification and purification of human type 2 lymphocyte (Th2) subsets.
Overview
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.
Data/Specifications
Clone: B4E6
IgG Subclass: IgG1
Form: The fluorescein (FITC) conjugated antibody is supplied in PBS and purified over protein Gsepharose from concentrated tissue culture supernatant. This antibody is supplied with 0.1% sodium azide as a preservative.
Specificity: This clone recognizes hST2L specifically on the cell surface of human type 2 (Th2, Tc2, NK2 and NKT2) but not type 1 lymphocytes.
Literature/Support
Monoclonal Antibody to ST2L, FITC (product insert)
Understanding The Four Isoforms of ST2 (blog post)
| References/Citations: | How the ST2L, FITC conjugated antibody was used: |
IL-33 Induces Antigen-Specific IL-5+ T Cells and Promotes Allergic-Induced Airway Inflammation Independent of IL-4 | Flow cytometry analysis to assess ST2 expression profile in cultured or freshly isolated T cells from drained lymph nodes (DLN) of BALB/c wild type mice and from CD4+ T cells of D0.11.10 mice. |
| Induction of IRAK-M Is Associated with Lipopolysaccharide Tolerance in a Human Endotoxemia Model Cornelis van ‘t Veer et al., J. Immunol., Nov 2007; 179: 7110 - 7120. | Flow cytometry analysis to assess the expression profile of ST2L in CD14high T cells from human whole blood collections. |
| The vast majority of CLA+ T cells are resident in normal skin. Clark R, et al. J of immunology., 2006; 176(7): 4431-9. | Flow cytometry analysis to examine CD4+ skin resident T cells for Th1/Th2 polarization (ST2L molecule is limited to Th2 cells). T cells were isolated from normal adult human skin obtained from cutaneous surgeries. Work performed on BD Biosciences FACScan instrument, data analyzed using CellQuest software. |
How To Use
Applications
- Flow cytometry
- FACS
- cell sorting
- immunohistochemistry with hybridoma culture supernatant