T1/ST2 (IL-33 R) Monoclonal Antibody, Biotinylated
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Mouse T1/ST2 (IL-33 R) biotinylated monoclonal antibody (clone DJ8) for the identification and purification of Th2 cells and all forms of murine mast cells.
Overview
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.
Data/Specifications
Rat anti-Mouse T1/ST2 biotinylated mAb
Clone: DJ8
Ig Subclass: IgG1 (light chain not isotyped)
Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.
Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine-stimulated murine fibroblasts.
Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.
Literature/Support
T1/ST2 Monoclonal Antibody, Biotinylated (PDF, product insert)
Understanding The Four Isoforms of ST2 (blog post)
References/Citations: | How the T1/ST2 Biotinylated antibody was used: |
| Cutting Edge: Atopy Promotes Th2 Responses to Alloantigens and Increases the Incidence and Tempo of Corneal Allograft Rejection Clay Beauregard et al., J. Immunol., Jun 2005; 174: 6577 - 6581. | Immunohistochemistry analysis to detect the presence of Th2 cells in four-micrometer cross sections of the eye. Eyes were obtained from C57/BL/6 and BALB/c mice. |
How To Use
This antibody allows the identification and purification of murine T helper 2 cells and all forms of murine mast cells.
Applications
- Flow cytometry
- immunoprecipitation