T1/ST2 (IL-33R) Monoclonal Antibody, FITC
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Mouse T1/ST2 (IL-33 R) FITC conjugated monoclonal antibody (clone DJ8) for the identification and purification of murine T helper 2 (Th2) cells and all forms of murine mast cells.
Overview
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.
Data/Specifications
Rat anti-Mouse T1/ST2 FITC-conjugated mAb
Clone: DJ8
Ig Subclass: IgG1 (light chain not isotyped)
Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.
Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine-stimulated murine fibroblasts.
Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.
Literature/Support
T1/ST2 Monoclonal Antibody, FITC (PDF, product insert)
Understanding The Four Isoforms of ST2 (blog post)
| References/Citations: | How the T1/ST2 FITC conjugated antibody was used: |
| Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses. Nakagawa, Y et al., J. Immunol. (2011) 186:2881 | CD4+ Tcells isolated from mice were stained with FITC conjugated anti-T1/ST2 antibodies and cultured. |
| Contribution of IL-33 to induction and augmentation of experimental allergic conjuctivitis. Matsuba-Kitamura S, et al., International Immunology, May 2010 | Immunohistochemistry analysis to detect the expression of ST2 in isolated conjunctival tissue from mice eyes. Used frozen tissue sections and examined expression patterns using confocal microscopy. |
| Eosinophils are dispensable for allergic remodeling and immunity in a model of house dust mite-induced airway disease. Fattouh R, et al. Am J Respir Crit Care Med. 2010 Aug 23. | |
| Contribution of IL-33 to induction and augmentation of experimental allergic conjuctivitis. Matsuba-Kitamura S, et al., International Immunology, May 2010 | Immunohistochemistry analysis to detect the expression of ST2 in isolated conjunctival tissue from mice eyes. Used frozen tissue sections and examined expression patterns using confocal microscopy. |
| Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of infection. Alves-Filho J, et al. Nature Medicine., 2010; 16(6):2-7. | Flow cytometry analysis to determine the expression of ST2 in bone marrow neutrophils isolated from BALB/c mice. |
| Activin-A induces regulatory T cells that suppress T helper cell immune responses and protect from allergic airway disease. Semitekolou M, et al. J. Exp. Med., 2009; 206(8):1769-85. | Flow cytometry analysis to determine the expression of T1/ST2 in cells isolated from lymph nodes of BALB/c mice. |
| IL-33 reduces the development of atherosclerosis Ashley M. Miller et al., J. Exp. Med., Feb 2008; 205: 339 - 346. | Flow cytometry analysis to determine the level of ST2 expression in lymph node cells isolated from mice. |
| IL-1beta-driven ST2L expression promotes maturation resistance in rapamycin-conditioned dendritic cells. Turnquist H, et al. Journ. Immunol., 2008; 181(1):62-72. | Flow cytometry analysis to detect the surface expression of ST2 in dendritic cells of mice. Analysis was performed using a LSR II flow cytometer and FlowJo software. |
| Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Xanthou G, et al. Nature Medicine. 2007; 13(5):570-80 | Flow cytometry analysis of T1/ST2 in isolated cells from draining lymph nodes of DO11.10 mice. |
| Soluble ST2 Blocks Interleukin-33 Signaling in Allergic Airway Inflammation Hiroko Hayakawa et al., J. Biol. Chem., Sep 2007; 282: 26369 - 26380. | Flow cytometry analysis to quantify the expression of ST2L in splenocytes isolated from mice and in murine thymoma EL-4 cells. |
| Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17 Susumu Nakae et al., J. Leukoc. Biol., May 2007; 81: 1258 - 1268. | Flow cytometry analysis to determine the expression of T1/ST2 on cells isolated from spleens of transgenic and wild-type mice. |
| Airway Epithelial STAT3 Is Required for Allergic Inflammation in a Murine Model of Asthma Marina C. Simeone-Penney et al., J. Immunol., May 2007; 178: 6191 - 6199. | Flow cytometry analysis to determine the percentage of Th2 cells in the lungs of mice. |
| CCR4 is a key modulator of innate immune responses. Ness T, et al. Journ. of immunol., 2006; 177(11): 7531-9. | Flow cytometry analysis to determine the expression of ST2 in peritoneal cells isolated from mice. Analysis was performed using a Beckman Coulter Cytomics FC 500 and FlowJo 6.3 software. |
IFN- Induces Apoptosis in Developing Mast Cells | Flow cytometry analysis to quantify the expression of T1/ST2 in bone marrow cells isolated from femurs and tibia of B6129PF1/J female mice. |
Identification of mast cell progenitors in adult mice | Flow cytometry analysis to detect the surface expression of T1/ST2 on mast cell progenitors (MCP). MCPs were isolated from the bone marrow of mice. |
How To Use
This antibody allows the identification and purification of murine T helper 2 cells and all forms of murine mast cells.
Applications
- Flow cytometry
- immunoprecipitation