Collagen Type I ELISA

collagen type I ELISA to measure type I collagen (CI) levels from human cells.
Catalog Number: 
M036007
Qty/Size: 
96 wells

collagen type I (CI)ELISA from MD Bioproducts

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The Human Collagen Type I (CI) ELISA kit is used to measure type I collagen levels from human cells.

Overview

Overview: 

Collagen Type I (CI) is the most abundant protein and is found in the skin, connective tissue, tendons, ligaments, cornea, invertebral discs and bone. It is encoded by the genes COL1A1 and COL1A2 and is formed inside (along the rough endoplasmic reticulum) and outside (by procollagen peptidase and bound by fibronectin and integrin) the cell. Individual collagen molecules are cross-linked to one another within these fibrils. The formation of cross-links results in very strong type I collagen fibrils, which are found in the spaces around cells.  Collagen Type I is involved in many human diseases such as fibrosis, osteoporosis, cancer and atherosclerosis.

 

Related Terms/Symbols:
 - COL-1
 - COL1A1
 - COL1A2
 - Alpha 1 type I collagen
 - Alpha 2 type I collagen
 - Collagen alpha 1 chain type I
 - Collagen type I alpha 1
 - Collagen type I alpha 2
 - Ol4
 - Osteogenesis imperfecta
 - Pro alpha 1 collagen type I
 - Type I procollagen
 - Ehlers-Danlos Syndrome
 - osteoporosis
 - pro-α2(I) chain
 - CO1A2_HUMAN

 

 

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  human cells

Sample Preparation: collagen must be solubilized

Sample Size: 100 uL

Standard Curve Range:  0.062 - 5 µg/mL

Sensitivity:  0.035 µg/mL

Assay Length:   <3 hrs

 

 

Literature/Support

Literature/Support: 

Product Insert:

Collagen Type I ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

The competitive assay (EIA) is a colormetric based immunoassay utilizing a biotinylated polyclonal antibody to Collagen Type I and purified human collagen type I as an antigen. Bound Collagen Type I coated on the surface of the microwells competes for the biotinylated antibody with free antigen in the standard and samples. This system creates an inverse relationship between OD values and concentration: the higher the OD value, the lower the concentration of collagen type I in the sample.

 

Collagen type I ELISA