Collagen Type II ELISA
Please register to view price.
Please inquire about bulk pricing.
The Collagen Type II (CII) ELISA kit is used to measure type II collagen levels in human, mouse, rat, bovine, porcine and chick cell culture supernate samples.
Overview
Type II Collagen (CII) is the principal collagenous component of cartilage, intervertebral disc and vitreous humour. It is encoded by the COL2A1 gene and its main function is to provide tensile strength and give cartilage the ability to resist shearing forces.
Symbols/Related Terms
- COL-2
- COL2A1
- pro-alpha 1(II) chain
- Achondrogenesis
- Hypochondrogenesis
- Spondyloepimetaphyseal dysplasia
- cartilage matrix
- Czech dysplasia
- Primary Osteoarthritis
- Stickler syndrome
- Kniest dysplasia
- Collagen Induced Arthritis (CIA)
- Rheumatoid Arthritis (RA)
- collagen, type II, alpha 1
- CO2A1_HUMAN
Data/Specifications
Species: human, mouse, rat, porcine, bovine, chick
Sample Type: cell culture supernate samples
Sample Preparation: collagen must be solubilized
Sample Size: 100 uL
Standard Curve Range: 4.7 - 300 ng/mL
Sensitivity: <2 ng/mL
Assay Length: <5 hrs
Literature/Support
Collagen Type II ELISA Insert (PDF)
| References/Citations: | How the Collage Type II ELISA Kit was used: |
| Intervertebral disc repair using adipose tissue-derived stem and regenerative cells: experiments in a canine model. Ganey T, et al. Spine. 2009; 34(21):2297-304. | Measure the concentration of type II collagen in intervertebral disc matrix samples obtained from the lumbar spine of canines. |
| Sustained hypoxia enhances chondrocyte matrix synthesis. Coyle CH, et al. J Orthop. Res. 2009 Jun;27(6):793-9. | Measure the concentration of type II collagen in primary bovine articular chondrocyte cultures. Chondrocytes were obtained from bovine knee cartilage samples. |
How It Works
ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a capture antibody
and a detection antibody to provide a unique and powerful assay system. The Collagen Type II ELISA is
a heterogeneous sandwich ELISA. A monoclonal anti-collagen type II antibody is pre-coated onto a 96-well
microplate and any collagen type II present in the sample will be bound onto the microplate by the antibody.
A secondary biotinylated antibody is then added and will bind to the immobilized antigen forming the
antibody-antigen complex. Substrate is then added and converted by the enzyme to produce a quantifiable
colored product that is in direct proportion to the amount of antigen bound in the initial reaction.