Mouse OVA-IgE ELISA

Mouse OVA-IgE ELISA
Catalog Number: 
M036005N
Qty/Size: 
96 wells

OVA-IgE ELISA from MD Bioproducts

North America: 1-888-USMDBIO
International: +41-44 986 2628

Or you can order by using our online form
Have questions or need bulk? No problem, just ask.

The Mouse OVA IgE ELISA Kit is used to measure OVA specific IgE levels in mouse serum and cell culture supernates.

Overview

Overview: 

IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.

 

IgE is well known for its involvement in eliciting an allergic or asthmatic response. Ovalbumin (OVA) is widely used for inducing an allergic response in mouse models of allergic asthma. Through T and B cell signalling, IgE production is stimulated and released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.

Data/Specifications

Data/Specifications: 

Species: mouse

Sample Type : cell culture supernate, serum 

Sample Preparation: dilute 1:10

Sample Size: 100 uL

Length: 2 hours 15 min 

Range: 7.8-500 ng/mL

Sensitivity: < 3.8 ng/mL

Literature/Support

Literature/Support: 

Product Insert:

Mouse OVA-IgE ELISA Insert (PDF, 276 KB)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

IgE mediated immune response in allergic asthma (blog post)

 

Liu, B., Lee, J. B., Chen, C. Y., Hershey, G. K. K., & Wang, Y. H. (2015). Collaborative Interactions between Type 2 Innate Lymphoid Cells and Antigen-Specific CD4+ Th2 Cells Exacerbate Murine Allergic Airway Diseases with Prominent Eosinophilia. The Journal of Immunology, 194(8), 3583-3593.

 

Aven, L., Paez-Cortez, J., Achey, R., Krishnan, R., Ram-Mohan, S., Cruikshank, W. W.,   & Ai, X. (2014). An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity. The FASEB Journal, 28(2), 897-907.

 

Arakawa, T., Deguchi, T., Sakazaki, F., Ogino, H., Okuno, T., & Ueno, H. (2013). Supplementary seleno-L-methionine suppresses active cutaneous anaphylaxis reaction. Biological and Pharmaceutical Bulletin, 36(12), 1969-1974.

 

Reddy, A. T., Lakshmi, S. P., Dornadula, S., Pinni, S., Rampa, D. R., & Reddy, R. C. (2013). The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease. The Journal of Immunology, 191(5), 2053-2063.

 

Paez-Cortez, J., Krishnan, R., Arno, A., Aven, L., Ram-Mohan, S., Patel, K. R., & Fine, A. (2013). A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.

 

Cloots, R. H., Sankaranarayanan, S., de Theije, C. C., Poynter, M. E., Terwindt, E., van Dijk, P.,   & Koehler, S. E. (2013). Ablation of Arg1 in hematopoietic cells improves respiratory function of lung parenchyma, but not that of larger airways or inflammation in asthmatic mice. American Journal of Physiology-Lung Cellular and Molecular Physiology, 305(5), L364-L376.

 

 

Knolle, M. D., Nakajima, T., Hergrueter, A., Gupta, K., Polverino, F., Craig, V. J., & Owen, C. A. (2013). Adam8 limits the development of allergic airway inflammation in mice. The Journal of Immunology, 190(12), 6434-6449.

 

Mays, L. E., Ammon-Treiber, S., Mothes, B., Alkhaled, M., Rottenberger, J., Müller-Hermelink, E. S., & Kormann, M. S. (2013). Modified Foxp3 mRNA protects against asthma through an IL-10–dependent mechanism. The Journal of clinical investigation, 123(3), 1216.

 

McKee, A. S., Burchill, M. A., Munks, M. W., Jin, L., Kappler, J. W., Friedman, R. S., & Marrack, P. (2013). Host DNA released in response to aluminum adjuvant enhances MHC class II-mediated antigen presentation and prolongs CD4 T-cell interactions with dendritic cells. Proceedings of the National Academy of Sciences, 110(12), E1122-E1131.

 

Asosingh, K., Cheng, G., Xu, W., Savasky, B. M., Aronica, M. A., Li, X., & Erzurum, S. C. (2013). Nascent endothelium initiates Th2 polarization of asthma. The Journal of Immunology, 190(7), 3458-3465.

 

Lombardi, V., Speak, A. O., Kerzerho, J., Szely, N., & Akbari, O. (2012). CD8α&plus; β− and CD8α&plus; β&plus; plasmacytoid dendritic cells induce Foxp3&plus; regulatory T cells and prevent the induction of airway hyper-reactivity. Mucosal immunology, 5(4), 432-443.

 

Nkyimbeng-Takwi, E. H., Shanks, K., Smith, E., Iyer, A., Lipsky, M. M., Detolla, L. J., & Chapoval, S. P. (2012). Neuroimmune semaphorin 4A downregulates the severity of allergic response. Mucosal immunology, 5(4), 409-419.

 

MacSharry, J., O'Mahony, C., Shalaby, K. H., Sheil, B., Karmouty-Quintana, H., Shanahan, F., & Martin, J. G. (2012). Immunomodulatory effects of feeding with Bifidobacterium longum on allergen-induced lung inflammation in the mouse. Pulmonary pharmacology & therapeutics, 25(4), 325-334.

 

Kim, S. R., Lee, K. S., Lee, K. B., & Lee, Y. C. (2012). Recombinant IGFBP‐3 inhibits allergic lung inflammation, VEGF production, and vascular leak in a mouse model of asthma. Allergy, 67(7), 869-877.

 

Emo, J., Meednu, N., Chapman, T. J., Rezaee, F., Balys, M., Randall, T., ... & Georas, S. N. (2012). Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation. The Journal of Immunology, 188(8), 3784-3790.

 

Diesner, S. C., Olivera, A., Dillahunt, S., Schultz, C., Watzlawek, T., Förster-Waldl, E., ... & Rivera, J. (2012). Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. Immunology letters, 141(2), 210-219.

 

Carr, V. M., Robinson, A. M., & Kern, R. C. (2012). Tissue-specific effects of allergic rhinitis in mouse nasal epithelia. Chemical senses, bjs048.

 

Singh, S. P., Gundavarapu, S., Peña-Philippides, J. C., Mishra, N. C., Wilder, J. A., Langley, R. J., ... & Sopori, M. L. (2011). Prenatal secondhand cigarette smoke promotes Th2 polarization and impairs goblet cell differentiation and airway mucus formation. The Journal of Immunology, 187(9), 4542-4552.

 

Reisacher, W. R., Liotta, D., Yazdi, S., & Putnam, D. (2011, September). Desensitizing mice to ovalbumin through subcutaneous microsphere immunotherapy (SMITH). In International forum of allergy & rhinology (Vol. 1, No. 5, pp. 390-395). Wiley Subscription Services, Inc., A Wiley Company.

 

MacNeil, A. J., Yang, Y. J., & Lin, T. J. (2011). MAPK Kinase 3 Specifically Regulates FcεRI-Mediated IL-4 Production by Mast Cells. The Journal of Immunology, 187(6), 3374-3382.

 

Stemmy, E. J., Balsley, M. A., Jurjus, R. A., Damsker, J. M., Bukrinsky, M. I., & Constant, S. L. (2011). Blocking cyclophilins in the chronic phase of asthma reduces the persistence of leukocytes and disease reactivation. American journal of respiratory cell and molecular biology, 45(5), 991-998.

 

Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.

 

Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.

 

Balsley, M. A., Malesevic, M., Stemmy, E. J., Gigley, J., Jurjus, R. A., Herzog, D., & Constant, S. L. (2010). A cell-impermeable cyclosporine A derivative reduces pathology in a mouse model of allergic lung inflammation. The Journal of Immunology, 185(12), 7663-7670.

 

Lauenstein, H. D., Quarcoo, D., Plappert, L., Schleh, C., Nassimi, M., Pilzner, C. & Groneberg, D. A. (2011). Pituitary adenylate cyclase‐activating peptide receptor 1 mediates anti‐inflammatory effects in allergic airway inflammation in mice. Clinical & Experimental Allergy, 41(4), 592-601.

 

Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., ... & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.

 

Lyons, A., O'Mahony, D., O'Brien, F., MacSharry, J., Sheil, B., Ceddia, M., & O'Mahony, L. (2010). Bacterial strain‐specific induction of Foxp3+ T regulatory cells is protective in murine allergy models. Clinical & Experimental Allergy, 40(5), 811-819.

 

Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.

 

Breslow, R. G., Rao, J. J., Xing, W., Hong, D. I., Barrett, N. A., & Katz, H. R. (2010). Inhibition of Th2 adaptive immune responses and pulmonary inflammation by leukocyte Ig-like receptor B4 on dendritic cells. The journal of immunology, 184(2), 1003-1013.

 

Yadav, U. C., Naura, A. S., Aguilera-Aguirre, L., Ramana, K. V., Boldogh, I., Sur, S., & Srivastava, S. K. (2009). Aldose reductase inhibition suppresses the expression of Th2 cytokines and airway inflammation in ovalbumin-induced asthma in mice. The Journal of Immunology, 183(7), 4723-4732.

 

Park, S. J., Lee, K. S., Kim, S. R., Min, K. H., Choe, Y. H., Moon, H., ... & Lee, Y. C. (2009). Peroxisome proliferator-activated receptor γ agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation. The Journal of Immunology, 183(5), 3259-3267.

 

Larsen, S. T., Roursgaard, M., Jensen, K. A., & Nielsen, G. D. (2010). Nano titanium dioxide particles promote allergic sensitization and lung inflammation in mice. Basic & clinical pharmacology & toxicology, 106(2), 114-117.

 

Lee, C. G., Hartl, D., Lee, G. R., Koller, B., Matsuura, H., Da Silva, C. A., ... & Elias, J. A. (2009). Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis. The Journal of experimental medicine, 206(5), 1149-1166.

 

Beigelman, A., Gunsten, S., Mikols, C. L., Vidavsky, I., Cannon, C. L., Brody, S. L., & Walter, M. J. (2009). Azithromycin attenuates airway inflammation in a noninfectious mouse model of allergic asthma. CHEST Journal, 136(2), 498-506.

 

References/Citations:Application:
Tissue-specific effects of allergic rhinitis in mouse nasal epithelia
Carr, V.M. et al., Chem Senses, Sep 2012; 37: 655 - 668.
Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchased from MD Bioproducts.
Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation
Emo, J et al., J. Immunol., Apr 2012; 188: 3784 - 3790
Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchased from MD Bioproducts.
Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis
Wang, W. et al., Otolaryngology -- Head and Neck Surgery, Feb 2011
Measure concentration of OVA-IgE from Balb/c mice with either PPAR-γ agonist pioglitazone (30 mg/kg/d) or pioglitazone plus PPAR-γ antagonist GW9662 (0.5 mg/d).
A Cell-Impermeable Cyclosporine A Derivative Reduces Pathology in a Mouse Model of Allergic Lung Inflammation
Balsley, M et al., J. Immunol., Dec 2010; 185: 7663
Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchased from MD Bioproducts.
Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells
Breslow, R et al., J. Immunol., Jan 2010; 184: 1003 - 1013.
Measure the concentration of serum IgE in genetically modified female mice of the BALB/c background (6-12 weeks old).
A Novel Dithiol Amide CB3 attenuates allergic airway disease through negative regulation of p38 MAPK
Kim SR, et al. Am. J. Respir. Crit. Care Med. April 2010
Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchased from MD Bioproducts.
Aldose Reductase Inhibition Suppresses the Expression of Th2 Cytokines and Airway Inflammation in Ovalbumin-Induced Asthma in Mice
Umesh C. S. Yadav et al., J. Immunol., Oct 2009; 183: 4723 - 4732.
Measure the concentration of OVA-IgE in broncheoalveolar lavage (BAL) fluid from C57BL/6 mice.

Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis
Chun Geun Lee et al., J. Exp. Med., May 2009; 206: 1149 - 1166.

Measure the concentration of OVA-IgE in splenocytes isolated from BALB/c WT and mutant mice.
Peroxisome Proliferator-Activated Receptor Agonist Down-Regulates IL-17 Expression in a Murine Model of Allergic Airway Inflammation
Seoung Ju Park et al, J. Immunol., Sep 2009; 183: 3259 - 3267.
 
Azithromycin Attenuates Airway Inflammation in a Noninfectious Mouse Model of Allergic Asthma
Avraham Beigelman et al., Chest, Aug 2009; 136: 498 - 506.
Measure the concentration of serum specific OVA-IgE in 7-week old BALB/cJ female mice.


 

How It Works

How It Works: 

ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. Antibody/antigen reactions take place on the suface of microplate wells that have been previously coated with a monoclonal antibody to mouse IgE heavy chain. Biotinylated ovalbumin and streptavidin-peroxidase, in the presence of substrate quantifies the analyte bound.

 

Assay Principle:

 

ELISA Assay Principle