OVA-IgE ELISA
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The OVA IgE ELISA Kit is used to measure OVA specific IgE levels in mouse serum and cell culture supernates.
Overview
IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.
IgE is well known for its involvement in eliciting an allergic or asthmatic response. Ovalbumin (OVA) is widely used for inducing an allergic response in mouse models of allergic asthma. Through T and B cell signalling, IgE production is stimulated and released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.
Data/Specifications
Species: mouse
Sample Type : cell culture supernate, serum
Sample Preparation: dilute 1:10
Sample Size: 100 uL
Length: 2 hours 15 min
Range: 7.8-500 ng/mL
Sensitivity: < 3.8 ng/mL
Literature/Support
Mouse OVA-IgE ELISA Insert (PDF, 276 KB)
IgE mediated immune response in allergic asthma (blog post)
| References/Citations: | Application: |
| Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis Wang, W. et al., Otolaryngology -- Head and Neck Surgery, Feb 2011 | Measure concentration of OVA-IgE from Balb/c mice with either PPAR-γ agonist pioglitazone (30 mg/kg/d) or pioglitazone plus PPAR-γ antagonist GW9662 (0.5 mg/d). |
| A Cell-Impermeable Cyclosporine A Derivative Reduces Pathology in a Mouse Model of Allergic Lung Inflammation Balsley, M et al., J. Immunol., Dec 2010; 185: 7663 | Serum from individual animals was analyzed for the presence of OVA-specific IgE using an ELISA kit purchased from MD Bioproducts. |
| Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells Breslow, R et al., J. Immunol., Jan 2010; 184: 1003 - 1013. | Measure the concentration of serum IgE in genetically modified female mice of the BALB/c background (6-12 weeks old). |
| A Novel Dithiol Amide CB3 attenuates allergic airway disease through negative regulation of p38 MAPK Kim SR, et al. Am. J. Respir. Crit. Care Med. April 2010 | |
| Aldose Reductase Inhibition Suppresses the Expression of Th2 Cytokines and Airway Inflammation in Ovalbumin-Induced Asthma in Mice Umesh C. S. Yadav et al., J. Immunol., Oct 2009; 183: 4723 - 4732. | Measure the concentration of OVA-IgE in broncheoalveolar lavage (BAL) fluid from C57BL/6 mice. |
Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis | Measure the concentration of OVA-IgE in splenocytes isolated from BALB/c WT and mutant mice. |
| Peroxisome Proliferator-Activated Receptor Agonist Down-Regulates IL-17 Expression in a Murine Model of Allergic Airway Inflammation Seoung Ju Park et al, J. Immunol., Sep 2009; 183: 3259 - 3267. | |
| Azithromycin Attenuates Airway Inflammation in a Noninfectious Mouse Model of Allergic Asthma Avraham Beigelman et al., Chest, Aug 2009; 136: 498 - 506. | Measure the concentration of serum specific OVA-IgE in 7-week old BALB/cJ female mice. |
How It Works
ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. Antibody/antigen reactions take place on the suface of microplate wells that have been previously coated with a monoclonal antibody to mouse IgE heavy chain. Biotinylated ovalbumin and streptavidin-peroxidase, in the presence of substrate quantifies the analyte bound.
Assay Principle:
