T1/ST2 ELISA, Mouse
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The Mouse T1/ST2 ELISA kit is used to measure T1/ST2 (IL-1 R4) levels in mouse serum and cell culture supernate samples.
Overview
ST2, also known as IL-1 R4 and T1 is an interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages and cardio myocytes. It binds IL-33, a cytokine that is up regulated by inflammation or mechanical strain in smooth muscle cells, keratinocytes, airway epithelia and cardiac fibroblasts. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma.
Related Terms/Symbols:
- T1/ST2 receptor
- IL-33 receptor chain (IL-33R)
- ST2 protein
- Interleukin 1 receptor-like 1 (IL1RL1 gene)
- ST2L
- ST2/Fit-1
- Interleukin-1 receptor 4 (IL-1 R4)
- DER4
- T1
- Q01638 (ILRL1_HUMAN)
Data/Specifications
Species: mouse
Sample Type : cell culture supernate, serum
Sample Preparation: neat (non-diluted)
Sample Size: 100 uL
Length: 2 hours 45 min (does not include read time)
Range: 0.156 to 10 ng/mL
Sensitivity: < 0.05 ng/mL
Literature/Support
How It Works
The Mouse T1/ST2 ELISA or Enzyme-Linked ImmunoSorbent Assay is a colormetric based immunoassay utilizing a monoclonal antibody to the membrane anchored T1M protein, bound on the surface of microwells as a capture antibody and a biotinylated Goat anti-Mouse polyclonal antibody for detection. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound. The assay is reduced using a Four Parameter curve fit.