Total IgE ELISA
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The Total IgE ELISA kit is used to measure total IgE levels in mouse serum samples.
Overview
IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.
IgE is well known for its involvement in eliciting an allergic or asthmatic response. After sensitization with an allergan, through T and B cell signalling stimulates IgE production. IgE is released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.
Data/Specifications
Species: mouse
Sample Type : serum
Sample Preparation: dilute at least 1:100
Sample Size: 100 uL
Length: 2 hours 15 min
Range: 15.6-500 ng/mL
Sensitivity: < 2.0 ng/mL
Literature/Support
Total IgE ELISA Product Insert (PDF, 276 KB)
IgE mediated immune response in allergic asthma (blog post)
How It Works
ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a monoclonal antibody to mouse IgE bound on the surface of microwells as a capture antibody. HRP-conjugated IgE in the presence of substrate is used as a detection antibody.
Assay Principle:
