Uromodulin (UMOD) ELISA
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The Uromodulin (UMOD), also known as Tamm-Horsfall (THP), ELISA kit provides an easy and rapid method for quantitatively measuring Uromodulin (THP) levels in mammalian urine samples.
Overview
Uromodulin (UMOD, also known as Tamm-Horsfall or THP) is the most abundant protein found in normal mammalian urine. Uormodulin is a GPI-anchored glycoprotein that is not derived from blood plasma but rather is produced by the thick ascending loop of Henle in the mammalian kidney. Until recently little has been known regarding the biological role of uromodulin (Tamm-Horsfall glycoprotein) in the kidney. It has been found that uromodulin, a biomarker for chronic kidney disease (CKD), could have other multi-faceted functions in the kidney. Recent studies have shown that variant genes in UMOD may play an important role in the regulation and risk for hypertension and cardiovascular disease. The protein expressed from the UMOD gene, uromodulin, may also have a functional role in sodium homeostasis in the kidney. These findings bring new insights to the role and pathophysiology of uromodulin and it’s association with hypertension and CKD.
Related Terms/Symbols:
- UMOD Gene
- THP
- THGP
- HNFJ
- ADMCKD2
- MCKD2
- FJHN
- uromucoid
- Tamm-horsfall glycoprotein
- P07911
Data/Specifications
Species: human
Cross-reactivity: canine
Sample Type : urine
Sample Preparation: dilute >1:200 (human), >1:50 (canine)
Sample Size: 50 uL
Length: 2 hours 40 min
Range: 2.344 – 150 ng/mL
Sensitivity: < 0.75 ng/mL
Literature/Support
Uromodulin ELISA Data Insert (PDF, 376 KB)
Urine Sample Preparation for Uromodulin Analysis
Uromodulin Biology and Pathophysiology (PDF journal article)
| References/Citations: | How the Uromodulin ELISA Kit was used: |
| Two-Dimensional Differential In-Gel Electrophoresis Proteomic Approaches Reveal Urine Candidate Biomarkers in Pediatric Obstructive Sleep Apnea David Gozal et al., Am. J. Respir. Crit. Care Med., Dec 2009; 180: 1253 - 1261 | The Uromodulin ELISA kit was used to measure Uromodulin levels in urine after morning collection from human subjects. |
How It Works
The Uromodulin ELISA is a colorimetric based sandwich immunoassay utilizing a polyclonal antibody to uromodulin bound on the surface of microwells as the capture antibody and a biotinylated polyclonal antibody to uromodulin as the detection antibody. When antigen is added to the well it is bound by the immobilized antibody. The biotinylated antibody then binds to the antigen forming and antigen-antibody complex. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound. Color development is directly proportional to the antigen concentration in the sample.
Sandwich Assay Principle:
