Human COMP ELISA

The human COMP ELISA kit is used to measure Cartlilage Oligomeric Matrix Protein
Catalog Number: 
M046055
Qty/Size: 
96 wells

Animal COMP ELISA from MD Bioproducts

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The Human COMP ELISA kit is used to measure Cartlilage Oligomeric Matrix Protein (COMP) levels in Human serum.

Overview

Overview: 

The Cartilage Oligomeric Matrix Protein (COMP) ELISA is a quantitative enzyme-linked immunosorbent assay for the determination of COMP in human serum. The presence of elevated levels, when considered in conjunction with other laboratory and clinical findings, is an aid in identifying aggressive destruction of joint tissue in diseases such as rheumatoid arthritis.

The human COMP molecule is a pentameric protein of five identical disulfide-linked subunits with a total molecular mass of 434 kDa. COMP was first described in cartilage but has recently also been found in tendons and synovial membranes. It has not been possible to detect COMP in culture medium from other connective tissues such as skin or lung.

When articular cartilage matrix is degraded by a disease process, protein fragments are produced that dif- fuse out into the joint fluid. Some of these proteins, such as COMP, subsequently appear in the bloodstream and can be used to monitor the progress of the cartilage degradation in inflammatory joint diseases such as rheumatoid arthritis and osteoarthritis. A quantitative relation has been shown between the concentrations of COMP in serum and cartilage degradation using radiographic changes as a surrogate clinical endpoint. This has been further proved in experimentally induced arthritis in animal models where the COMP serum level was highly correlated to the severity of arthritis and also the clinical joint score and histopathological signs of cartilage erosion.

 

 

Data/Specifications

Data/Specifications: 

Species: Human

Sample Type:  serum

Sample Preparation: dilute 1:10

Sample Size: 25 uL

Standard Curve Range:  up to ~3.5 U/L

Sensitivity:  <0.1 U/L

Assay Length:   <2.5 hrs

 

 

Literature/Support

Literature/Support: 

Product Insert:

Human COMP ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

  
  
  
  
  

How It Works

How It Works: 

The human COMP ELISA is a quantitative solid-phase enzyme-linked immunosorbent assay. It is based on the sandwich technique in which two monoclonal antibodies are directed against separate antigenic determi- nants on the COMP molecule. During incubation COMP in the sample reacts with peroxidase-conjugated an- ti-COMP antibodies (conjugate) and anti-COMP antibodies bound to the microplate wells. A simple washing step removes unbound conjugate. The bound conjugate is detected by a reaction with the enzyme substrate tetramethylbenzidine (TMB). This reaction is stopped by adding acid to give a colorimetric endpoint that is read spectrophotometrically.