Mouse IgG1 Isotype Control, low endotoxin, azide-free
Mouse IgG1 Isotype control, Low endotoxin, azide-free
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
The low endotoxin, azide-free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Store at 2 – 8 °C. Aliquot and store at or below -20°C.
How To Use
- Used for Flow cytometry
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA)
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA)
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs