Mouse IgG1 Isotype Control, Purified Antibody
Mouse IgG1 Isotype control, purified
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
This product supplied as 1.0 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2.
Store at 2 – 8 °C
How To Use
- Used for Flow cytometry: working dilution of ≤1 ug/106 cells
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA): working dilution of 1 – 5 ug/mL
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA).
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs