Rat IgG1 Isotype Control, FITC Conjugated Antibody
Rat IgG1 Isotype Control, FITC conjugate antibody
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Keyhole limpet hemocyanin (KLH)
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3.
Store at 2 – 8 °C
How To Use
- Used for Flow cytometry: working dilution ≤ 1 ug / 106 cells
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs