Rat IgG1 Isotype Control, Purified Antibody

Catalog Number: 
1053001
Qty/Size: 
0.5 mg

rat IgG1 isotype control  from MD Bioproducts

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Rat IgG1 Isotype Control Purified Antibody

Overview

Overview: 

Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).

Data/Specifications

Data/Specifications: 

Ig Isotype:
Rat IgG1k

Specifcity:
Keyhole limpet hemocyanin (KLH)

Form:
This product supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2.

Storage:
Store at 2 – 8 °C

 

Literature/Support

Literature/Support: 

How To Use

How To Use: 

Applications:

  • Used for Flow cytometry.
  • Used for Enzyme-Linked–Immunosorbent-Assay (ELISA): recommended dilution of 1 ug/mL
  • Used for Flourescent-Linked-Immunosorbent-Assay (FLISA).

Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs