Collagen Type I and III, canine
Purified type I & III canine collagen.
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
- Collagen type III, alpha 1
- osteogenesis imperfecta
Canine collagen type I and III - 98%
Canine Collagen (other types) - <1%
Non-collagen proteins - below 0.5%
Form: 10 mg lyophilized, salt-free
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III were purified by using differential salt precipitation.
Reconstitution: Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Note: inserts are for review only. Please refer to the insert shipped with your product to ensure the most up-to-date revision is being used.
How To Use
- Coating material for cell culture studies.
Note: May not be suitable for 3-D gel formation.