Collagen Type I, Mouse
Purified type I native murine collagen from mouse tail tendon.
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
- osteogenesis imperfecta
Reconstitution: Use 0.5 M acetic acid, pH 2.5 at 4°C. Collagen solubility 10 mg/ml. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: mouse tail tendon
Form: lyophilized, 1 mg/vial
Murine collagen Type I - 90%
Murine Collagen Type III - 10%
Other collagen types - <1%
Non-collagen proteins - <0.5%
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
- Used for coating material for cell culture studies.
Note: may not be appropriate for 3D Gel preparation