Collagen Type II, Bovine, Lyophilized
Please register to view price.
Bulk orders available. Please inquire here.
Collagen type II (CII) protein, purified from fetal bovine articular cartilage, for the induction of arthritis in the Collagen-Induced Arthritis (CIA) model.
Overview
Type II collagen (CII) is a fibrillar collagen that is primarily located in cartilage tissue. The major molecular form of collagen in cartilage is type II collagen. Type II collagen is also located in the vitreous humor of the eye, the inner ear, the nose, and the intervertebral discs of the spine. The function of type II collagen is to provide tensile strength and give cartilage the ability to resist shearing forces. Mutations of the COL2A1 gene affect the synthesis of type II collagen and cause chondrodysplasias. This is characterized by abnormal cartilage that leads to bone and joint deformities. The degradation of collagen type II is an early indicator of osteoarthritis.
Type II Collagen and Adjuvent Susceptibility to CIA is linked to MHC class II molecules and is dependent upon the species of type II collagen used for immunization. Various species of highly purified Type II Collagen are supplied lyophilized and in solution for use in the induction of arthritis in vivo.
Symbols/Related Terms:
- COL2A1
- pro-alpha 1(II) chain
- Achondrogenesis
- Hypochondrogenesis
- Spondyloepimetaphyseal dysplasia
- Osteoarthritis (OA)
- Collagen Induced Arthritis (CIA)
Data/Specifications
Concentration: Can be reconstituted to concentration of choice.
Purity: >99% as determined by SDS-PAGE and visualized by Coomassie blue staining
Purification: Differential salt precipitation
Source: Fetal bovine articular cartilage
Form: lyophilized
Literature/Support
Bovine Collagen Type II Insert (PDF)
References/Citations: | How the purified bovine collagen type II (bCII) protein was used: |
| Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis. Buatois V, et al. J immunol. 2010 Aug 15; 185(4):2544-54. | Induce arthritis in mice. |
| Liver X Receptor Agonism Promotes Articular Inflammation in Murine Collagen-Induced Arthritis Asquith DL, Miller AM, et al. Arthritis Rheum. 2009 Sep; 60(9):2655-65. | Induce arthritis in male DBA/1 mice at 7-8 weeks of age. On day 0, mice were injected intradermally with 100 ug of bCII/CFA emulsification. On day 21, 200 ug bCII/PBS was injected intraperitoneally. |
| Destructive Arthritis in the Absence of Both FcRI and FcRIII Peter Boross et al., J. Immunol., Apr 2008; 180: 5083 - 5091. | Induce arthritis in male mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA. A boost was given on day 28 with 100 ug of bCII emulsified in IFA. Additionally, bCII protein was used as a plate-coating reagent to create an ELISA to measure anti-bCII antibodies. |
| Efficient suppression of murine arthritis by combined anticytokine small interfering RNA lipoplexes. Khoury M, et al. Arthritis Rheum. 2008 Aug; 58(8):2356-67 | Induce arthritis in 9 week-old male DBA/1 mice. |
| Immunomodulatory Dendritic Cells Inhibit Th1 Responses and Arthritis via Different Mechanisms Leonie M. van Duivenvoorde et al., J. Immunol., Aug 2007; 179: 1506 - 1515. | Induce arthritis in male DBA/1J mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA. A boost was given three weeks later with 100 ug of bCII emulsified in IFA. |
| Antibody-mediated delivery of IL-10 inhibits the progression of established collagen-induced arthritis. Trachsel E, et al. Arthritis Res Ther. 2007;9(1):R9 | Induce arthritis in male DBA/1 mice (8-12 weeks' old). Mice were immunized at the tail base with 200 ug of bCII emulsified in CFA. A boost was given two weeks later with 200 ug of bCII emulsified in IFA. |
The non-thiol angiotensin-converting enzyme inhibitor quinapril suppresses inflammatory arthritis | Induce arthritis in adult male DBA/101aHsd mice. Mice were immunized at the base of the tail with 100 ug of bCII emulsified in CFA. |
| Inflammatory arthritis and dermatitis in thymectomized, CD25+ cell-depleted adult mice A. Loughry et al., Rheumatology, Mar 2005; 44: 299 - 308. | Induce arthritis in male DBA/1j mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA. |
Tracking of Proinflammatory Collagen-Specific T Cells in Early and Late Collagen-Induced Arthritis in Humanized Mice | Induce arthritis in genetically modified mice on a DBA1/J background. Mice were immunized at the tail base with 100 - 150 ug of bCII emulsified in CFA. A boost was given on day 40 with 50 - 100 ug of CII emulsified in IFA. |
How To Use
Key Applicatons:
- Induction of arthritis in vivo
- Attachment and differentiation of chondrocytes
- Cell culture
- ELISA standard/plate coating