Collagen Type II, Bovine, Lyophilized
Collagen type II (CII) protein, purified from fetal bovine articular cartilage, for the induction of arthritis in the Collagen-Induced Arthritis (CIA) model.
Type II collagen (CII) is a fibrillar collagen that is primarily located in cartilage tissue. The major molecular form of collagen in cartilage is type II collagen. Type II collagen is also located in the vitreous humor of the eye, the inner ear, the nose, and the intervertebral discs of the spine. The function of type II collagen is to provide tensile strength and give cartilage the ability to resist shearing forces. Mutations of the COL2A1 gene affect the synthesis of type II collagen and cause chondrodysplasias. This is characterized by abnormal cartilage that leads to bone and joint deformities. The degradation of collagen type II is an early indicator of osteoarthritis.
Type II Collagen and Adjuvent Susceptibility to CIA is linked to MHC class II molecules and is dependent upon the species of type II collagen used for immunization. Various species of highly purified Type II Collagen are supplied lyophilized and in solution for use in the induction of arthritis in vivo.
- pro-alpha 1(II) chain
- Spondyloepimetaphyseal dysplasia
- Osteoarthritis (OA)
- Collagen Induced Arthritis (CIA)
Concentration: Can be reconstituted to concentration of choice.
Purity: >99% as determined by SDS-PAGE and visualized by Coomassie blue staining
Purification: Differential salt precipitation
Source: Fetal bovine articular cartilage
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|How the purified bovine collagen type II (bCII) protein was used:|
|The TNF family member APRIL dampens collagen-induced arthritis. Fernandez,L et al.(2012) Ann Rheum Dis doi:10.1136/annrheumdis-2012-202382||Arthritis was induced in APRIL-Tg DBA/1 mice using a 1:1 emulsion of bovine CII and CFA. on day 21, a CII boost was given. Clinical scores were monitored to day 60.|
|TGF-β–Exposed Plasmacytoid Dendritic Cells Participate in Th17 Commitment|
Bonnefoy, F et al., J Immunol., Jun 2011; 166:6157.
|DBA-1 mice were immunized with 100 uL bovine type II collagen (200 ug/mL)|
|A Virus-Like Particle-Based Anti-Nerve Growth Factor Vaccine Reduces Inflammatory Hyperalgesia: Potential Long-Term Therapy for Chronic Pain|
Rohn, T et al., J. Immunol., Feb 2011; 186: 1769
|RA was induced by intradermal injection of collagen type II in CFA followed by an intradermal injection of collagen type II in IFA 21 d later.|
|Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis.|
Buatois V, et al. J immunol. 2010 Aug 15; 185(4):2544-54.
|Induce arthritis in mice.|
|Liver X Receptor Agonism Promotes Articular Inflammation in Murine Collagen-Induced Arthritis|
Asquith DL, Miller AM, et al. Arthritis Rheum. 2009 Sep; 60(9):2655-65.
|Induce arthritis in male DBA/1 mice at 7-8 weeks of age. On day 0, mice were injected intradermally with 100 ug of bCII/CFA emulsification. On day 21, 200 ug bCII/PBS was injected intraperitoneally.|
|Destructive Arthritis in the Absence of Both FcRI and FcRIII|
Peter Boross et al., J. Immunol., Apr 2008; 180: 5083 - 5091.
|Induce arthritis in male mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA. A boost was given on day 28 with 100 ug of bCII emulsified in IFA. Additionally, bCII protein was used as a plate-coating reagent to create an ELISA to measure anti-bCII antibodies.|
|Efficient suppression of murine arthritis by combined anticytokine small interfering RNA lipoplexes.|
Khoury M, et al. Arthritis Rheum. 2008 Aug; 58(8):2356-67
|Induce arthritis in 9 week-old male DBA/1 mice.|
|Immunomodulatory Dendritic Cells Inhibit Th1 Responses and Arthritis via Different Mechanisms|
Leonie M. van Duivenvoorde et al., J. Immunol., Aug 2007; 179: 1506 - 1515.
Induce arthritis in male DBA/1J mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA. A boost was given three weeks later with 100 ug of bCII emulsified in IFA.
|Antibody-mediated delivery of IL-10 inhibits the progression of established collagen-induced arthritis.|
Trachsel E, et al. Arthritis Res Ther. 2007;9(1):R9
|Induce arthritis in male DBA/1 mice (8-12 weeks' old). Mice were immunized at the tail base with 200 ug of bCII emulsified in CFA. A boost was given two weeks later with 200 ug of bCII emulsified in IFA. |
The non-thiol angiotensin-converting enzyme inhibitor quinapril suppresses inflammatory arthritis
|Induce arthritis in adult male DBA/101aHsd mice. Mice were immunized at the base of the tail with 100 ug of bCII emulsified in CFA.|
|Inflammatory arthritis and dermatitis in thymectomized, CD25+ cell-depleted adult mice|
A. Loughry et al., Rheumatology, Mar 2005; 44: 299 - 308.
|Induce arthritis in male DBA/1j mice. Mice were immunized at the tail base with 100 ug of bCII emulsified in CFA.|
Tracking of Proinflammatory Collagen-Specific T Cells in Early and Late Collagen-Induced Arthritis in Humanized Mice
|Induce arthritis in genetically modified mice on a DBA1/J background. Mice were immunized at the tail base with 100 - 150 ug of bCII emulsified in CFA. A boost was given on day 40 with 50 - 100 ug of CII emulsified in IFA.|
How To Use
- Induction of arthritis in vivo
- Attachment and differentiation of chondrocytes
- Cell culture
- ELISA standard/plate coating