Collagen Type II, Chicken, Lyophilized
Collagen type II (CII) protein, purified from chicken sternum, for the induction of arthritis in the Collagen-Induced Arthritis (CIA) model.
Type II collagen (CII) is a fibrillar collagen that is primarily located in cartilage tissue. The major molecular form of collagen in cartilage is type II collagen. Type II collagen is also located in the vitreous humor of the eye, the inner ear, the nose, and the intervertebral discs of the spine. The function of type II collagen is to provide tensile strength and give cartilage the ability to resist shearing forces. Mutations of the COL2A1 gene affect the synthesis of type II collagen and cause chondrodysplasias. This is characterized by abnormal cartilage that leads to bone and joint deformities. The degradation of collagen type II is an early indicator of osteoarthritis.
Type II Collagen and Adjuvent Susceptibility to CIA is linked to MHC class II molecules and is dependent upon the species of type II collagen used for immunization. Various species of highly purified Type II Collagen are supplied lyophilized and in solution for use in the induction of arthritis in vivo.
- pro-alpha 1(II) chain
- Spondyloepiphyseal dysplasia
- Osteoarthritis (OA)
- Collagen Induced Arthritis (CIA)
Concentration: Can be reconstituted to concentration of choice.
Purity: >99% as determined by SDS-PAGE and visualized by Coomassie blue staining
Purification: Differential salt precipitation
Source: chick sternum
Roy, L. D., Sahraei, M., Schettini, J. L., Gruber, H. E., Besmer, D. M., & Mukherjee, P. (2014). Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches. BMC cancer, 14(1), 225.
Chen, Y. M., Chuang, H. C., Lin, W. C., Tsai, C. Y., Wu, C. W., Gong, N. R., ... & Chen, D. Y. (2013). Germinal Center Kinase–like Kinase Overexpression in T Cells as a Novel Biomarker in Rheumatoid Arthritis. Arthritis & Rheumatism, 65(10), 2573-2582.
Marenzana, M., Vugler, A., Moore, A., & Robinson, M. (2013). Effect of sclerostin-neutralising antibody on periarticular and systemic bone in a murine model of rheumatoid arthritis: a microCT study. Arthritis Res Ther, 15(5), R125.
Iqbal, A. J., Cooper, D., Vugler, A., Gittens, B. R., Moore, A., & Perretti, M. (2013). Endogenous galectin-1 exerts tonic inhibition on experimental arthritis. The Journal of Immunology, 191(1), 171-177.
Vierboom, M., Breedveld, E., Kondova, I., & 't Hart, B. (2010). Collagen-induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis. Arthritis Research and Therapy, 12(5), R200.
Asquith, D. L., Miller, A. M., Hueber, A. J., Liew, F. Y., Sattar, N., & McInnes, I. B. (2010). Apolipoprotein E–deficient mice are resistant to the development of collagen‐induced arthritis. Arthritis & Rheumatism, 62(2), 472-477.
|References/Citations:||How the Chicken Collagen Type II protein was used:|
|Apolipoprotein E-deficient mice are resistant to the development of collagen-induced arthritis.|
Asquith DL, Miller AM, et al. Arthritis Rheum. 2010 Feb; 62(2):472-7.
|Induce arthritis in C57BL/6 WT or ApoE(-/-) mice at 12 weeks of age. On day 0, mice were injected intradermally with 200 μg of type II chicken collagen with CFA. On day 21, a booster of type II chicken collage/PBS (200 μg) was injected intraperitoneally.|
|Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis. Van Beneden K, et al. Annals of the rheumatic diseases. 2009; 68(8):1360-5.||Induce arthritis in DBA/1 mice. A 100 uL emulsion was used for induction and animals were challenged with a secondary injection of CII in incomplete Freund adjuvant 21 days later.|
How To Use
- Induction of arthritis in vivo
- Cell Culture
- ELISA standard/plate coating