Collagen Type II Staining Kit
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Collagen type II kit for the detection of collagen type II in tissues or cells via immunoblotting or immunohistochemistry.
Overview
The collagen molcule is a unique structure composed of 3 alpha chains that form a triple helical structure.
There are 26 unique type of collagen all with a unique amino acid sequence that causes the structure to
be stable, highly resistant to proteolytic degradation, heat, and chemicals. Since the different collagen
molecules are similar in structure and amino acid sequence, it is very difficult to identify them by ordinary
chemical or histological evaluations. To aid with that, this staining kit provides a cocktail of monoclonal
antibodies for type II collagen. The epitope specificity of this antibody cocktail allows for immunoblotting or
immunostaining of collagen type II in tissue.
Data/Specifications
Kit Components:
Collagen type II monoclonal antibody - Biotinylated monoclonal antibody to collagen type
II.
Antibody Dilution Buffer - 1 vial of buffer containing sodium azide.
Blocking Buffer - 1 vial of a protein buffer.
Streptavidin-HRP Concentrate - 1 vial of 100-fold concentrated Streptavidin-HRP in a
stabilizing buffer.
HRP Diluent - 1 vial of a protein buffer.
Literature/Support
Collagen Type II Staining Kit Product Insert (pdf)
| References/citations: | How the collagen type II staining kit was used: |
| The carbon monoxide-releasing molecule tricarbonyldichlororuthenium(II) dimer protects human osteoarthritic chondrocytes and cartilage form the catabolic actions of interleuikin-1B. Megias J, Guillen MI, Bru A, et al. (2008) | The type II collagen staining kit was used in immunocytochemistry analysis to detect collagen type II expression in chondrocytes in primary culture. Cells were fixed with 4% formaldehyde in phosphate-buffered saline for 30 minutes at 4°C. |