Aggrecan Western Blot Kit
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Aggrecan Western blot kit for the detection of aggrecanase (ADAMTS-1, -4 & -5) generated N-terminal neoepitope ARG Metabolites in the IGD domain of Aggrecan by immunoblot analysis.
Overview
Western Blot analysis is used to detect your protein of interest from a mixture of a several proteins. By using Western Blots, you can gain information about the size of your protein and it’s expression.
Western blots can analyze any protein sample whether from cells or tissues. The analysis is dependent on the quality of antibody used to probe for your protein of interest and how specific it is for this protein.
Western Blot kits are fully optimized and come complete with:
- Nitrocellulose membranes (small or large)
- Primary Antibody
- Secondary Antibody
- Standard
- Substrate system
- Buffers
Data/Specifications
Clone: BC3
Length: 3 hours
Sensitivity: < 2 ug
Specificity: Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognize the sequence ARG if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognize the aggrecanase generated neoepitope ARG.
Cross-reactivity: human, rat, cat, cow, dog, guinea pig, horse, pig, rabbit and sheep.
Membrane size:
- large membrane is 85 mm x 135 mm, 0.2 um
- Small membrane is 75 mm x 85 mm, 0.2 um