Cartilage-link protein (HAPLN1) monoclonal antibody (clone 8A4).
Overview
Overview:
Cartilage-link protein (LP) is a glycoprotein present in cartilage that stabilizes the interaction of aggrecan (versican) with hyaluronic acid (HA) in large proteoglycan aggregates.
Related Terms/Symbols:
- Anti-HAPLN1 antibody
- CRTL1
- Hyaluronan and proteoglycan link protein 1
- LP
- Proteoglycan link protein
- Cartilage-linking protein 1
- HAPLN1 gene
- P10915
Data/Specifications
Data/Specifications:
Immunogen: Link Protein from Bovine nasal cartilage
Clone: 8A4
Host: Mouse
Isotype: IgG
Specificity: This antibody recognizes the structurally related amino acid sequence epitopes … PISRP… and ….PITKP that occur in the two proteoglycan tandem repeat motifs of cartilage LP. This antibody can be used to detect intact LP 1,2 & 3 as well as a large number of smaller fragments that are generated through proteolytic cleavage during LP catabolism. It recognizes LP in all animal species from chicken to humans.
Purity: Affinity purified
Form: Clear liquid
Concentration: 0.1 mg/mL
Storage: -20° C
How To Use
How To Use:
Applications
Western Blot
IHC
RIA
Technical Notes
No enzyme pretreatment required, however chondroitinase and hyaluronidase will increase antibody sensitivity by exposing epitopes. For WB chondroitinase and hyaluronidase treatment is recommended.