Endorphin

Endorphin (β-Endorphin) ELISA

Catalog Number: 
M056011
Qty/Size: 
96 wells

Please inquire about bulk pricing.

The β-endorphin ELISA kit is designed to measure β-Endorphin levels in human samples.

Overview

Overview: 

β-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. It is a 31 amino acids long resulting from processing of the precursor proopiomelanocortin (POMC), which also gives rise to the other peptide hormones including ACTH (adrenocorticotropic hormone) and α- and γ-MSH (melanocyte-stimulating hormone). β-endorphin is an agonist of opioid receptors giving it analgesic properties. 

 

 

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma

Sample Preparation: plasma extraction

Sample Size: 50 uL

Standard Curve Range:  0.01 - 100 ng/mL

Sensitivity:  1 ng/mL

Assay Length:   4 hrs

Cross-reactivity:

Leu-Enkephalin: 0 %

MET-Enkephalin: 0 %

beta_Endorphin (Human): 100 %

alpha Endorphin: 0 %

Gamma Endorphin: 0 %

Literature/Support

Literature/Support: 

β-endorphin ELISA Insert (PDF)

 

References/Citations:The Beta-Endorphin ELISA Kit was used for:
Validation of a sham acupuncture procedure in a randomised, controlled clinical trial of chronic pelvic pain treatment
Lee, SW et al., Acupunct Med. 2011
Measure the concentration of beta-endorphin in accupuncture treated patient group.
Vernonia cinerea Less. supplementation and strenuous exercise reduce smoking rate: relation to oxidative stress status and beta-endorphin release in active smokers.
Leelarungrayub D, et al. J Int Soc Sports Nutr. 2010 May; 7:21.
Measure the concentration of beta-endorphin in human plasma samples.
Propofol/alfentanil and propofol/ketamine procedural sedation in children with acute lymphoblastic leukaemia: safety, efficacy and their correlation with pain neuromediator expression.
Chiaretti A, et al. Eur J Cancer Care. 2010 Mar; 19(2):212-20.
 

Endothelin-A Receptor Antagonism Attenuates Carcinoma-Induced Pain Through Opioids in Mice.
Quang PN and Schmidt BL. J Pain. 2010 Jan (Epub ahead of print)

Measure the concentration of BE in media collected from squamous cell carcinoma (SCC) cell culture. The SCC cell line was derived from human tongue.
The Effects of Hydrotherapy on Anxiety, Pain, Neuroendocrine Responses, and Contraction Dynamics During Labor.
Benfield RD, et al. Biol Res Nurs. 2010 May
Measure the concentration of BE in plasma samples from female humans. Plasma was prepared from whole blood collected in vacutainers containing EDTA. Traysylol (5,000 KIU) was added to inhibit proteolysis. Blood was spun at 3,000 rpm for 15 minutes at 14 °C and the plasma was collected, stored at -80 °C.
Biologically active breast milk proteins in association with very preterm delivery and stage of lactation.
Mehta R and Petrova A. J Perinatol. 2010 Jun
Measure the concentration of beta-endorphin in breast milk samples obtained from female humans.
Serum beta-endorphin response to stress before and after operation under fentanyl anesthesia in neonates, infants and preschool children.
Mirilas P, et al. Eur J Pediatr Surg. 2010 Mar; 20(2):106-10.
Measure concentration of serum BE in human samples.
Clinical and endocrinological changes after electro-acupuncture treatment in patients with osteoarthritis of the knee.
Ahsin S, et al. Pain. 2009 Dec; 147(1-3):60-6.
Measure the concentration of beta-endorphin in plasma from human patients with osteoarthritis of the knee. Plasma was obtained from blood collected in a pre-cooled EDTA tube.
Peripheral endothelin B receptor agonist-induced antinociception involves endogenous opioids in mice.
Quang PN and Schmidt BL. Pain. 2010 May; 149(2):254-62.
Measure the concentration of BE in media collected from squamous cell carcinoma (SCC) cell culture. The SCC cell line was derived from human tongue.
Beta-endorphin in serum and seminal plasma in infertile men.
El-Hagger S, et al. Asian J Androl. 2006 Jun; 8(6):709-12.
Measure the concentration of beta-endorphin in seminal plasma and serum from male human patients.
Cognitive and emotional factors in placebo analgesia.
Flaten MA, et al. J Psychosom Res. 2006 Jul; 61(1):81-9.
Measure the concentration of serum BE from adult humans. Serum was prepared from whole blood by centrifugation at 1500g for 10 minutes.

Plasma beta-endorphin levels in frequent and infrequent tanners before and after ultraviolet and non-ultraviolet stimuli.
Kaur M, et al. J Am Acad Dermatol. 2006 May;54(5):919-20.

Measure the concentration of beta-endorphin in plasma from female human subjects exposed to Ultraviolet light.
Treatment with double dose of omezprazole increases Beta Endorphin plasma in patients with coronary artery disease.
Budzynski J, et al. Archives of Medical Science. 2010; 6(2):201-207.
 

 

 

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