Vitamin H

Vitamin H ELISA

Vitamin H ELISA
Catalog Number: 
M046019
Qty/Size: 
96 wells

vitamin H ELISA from MD Bioproducts

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The Vitamin H ELISA kit is intended to measure Vitamin H levels in human serum, plasma and urine.

Overview

Overview: 

Biotin, also known as vitamin H, is of great importance for the biochemistry of the human organism. As a prosthetic group of mitochondrial enzymes (carboylases), biotin plays a central role as a CO2-carrier in important metabolic reactions such as gluconeogenesis, synthesis of fatty acids and metabolism of amino acids. Furthermore, biotin influences the growth and maintenance of blood cells, sebaceous glands, skin, hair and nails. Next to the free form of biotin, the biotin linked to lysin, also known as biocytine, can also be utilized as a vitamin source by the body, after cleavage from the protein by the enzyme biotinidase.

 

In nature, biotin is very common. It can be found in bacteria and mushrooms, as well as in higher plant life and animal tissue - especially in liver and kidney. However, the biotin availability in some food is very slim. Amounts worth mentioning can only be found in yeast, soya beans, nuts, cauliflower, lentils, oats, wheat germ and in egg yolk whereas fruits, milk products and most vegetables only contain small amounts. Solely, the amount of biotin is not the decisive factor, but rather the bioavailability. The bioavailability varies very strongly and depends on the kind of food and also on the extent to which biotin is protein-bound. Whereas, for example, in plants, biotin is available in the free form, food coming from animals contains mostly protein-bound biotin. Only after proteolytic reduction in the small intestine followed by a cleavage with intestine (pancreas)-biotinidase is the biotin available in the free absorbable form.

 

Biotin which is synthesized endogenously by flora of the intestine is not reabsorbed in the colon, but is stored as protein-bound biotin in the bacteria of the intestine and is thus not available to cover the biotin requirement of the organism. The treatment of food also causes losses in biotin. Wheat, as a whole grain, contains 4 times as much biotin as all-purpose flour (Type 405). The alimentary utilization of biotin is estimated at 50 %, so that a biotin deficiency can easily result. Malnutrition as well as an inherited disorder in biotin metabolism (singular or multiple deficiency in carboxylase, biotinidase deficiency) can lead to a biotin deficiency. Furthermore, circumstances of life, where an increased biotin demand exists (pregnancy, nursing, athletic activities, pathological conditions) may cause a biotin malnutrition. A variety of disorders on hair, skin and nails are the medically relevant consequences of a biotin deficiency. The symptoms range from brittle, splintered fingernails to different forms of alopecia to scaly erythematous and seborrheic dermatitis. Animals have also been observed with similar illnesses, ranging from larger skin detachments, epidermal crust development, as well as a hyper- and parakeratosical change in mucous membrane.

 

 

 

 

Data/Specifications

Data/Specifications: 

Species: Human

Sample Type:  serum, plasma, urine

Sample Preparation: Dilute samples

Sample Size: 50 uL

Standard Curve Range:  12.3 - 1000 ng/L

Assay Length:   1.5 hrs

 

 

Literature/Support

Literature/Support: 

Product Insert:

Vitamin H ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

Publications/References:

Effect of dietary n-3 fatty acids (fish oils) on boar reproduction and semen quality.

Castellano CA, Audet I, Bailey JL, et al. J Anim. Sci. 2010 Jul; 88(7):2346-55.

 

Perry, C. A., West, A. A., Gayle, A., Lucas, L. K., Yan, J., Jiang, X., & Caudill, M. A. (2014). Pregnancy and lactation alter biomarkers of biotin metabolism in women consuming a controlled diet. The Journal of nutrition, 144(12), 1977-1984.

 

Castellano, C. A., Audet, I., Bailey, J. L., Chouinard, P. Y., Laforest, J. P., & Matte, J. J. (2010). Effect of dietary n-3 fatty acids (fish oils) on boar reproduction and semen quality. Journal of animal science, 88(7), 2346-2355.

How It Works

How It Works: 

The basis of the test is the extremely high affinity of avidin (a protein produced by microorganisms) to biotin. The microtiter plates are coated with avidin. The enzyme-labeled biotin (Conjugate) and the sample or the Biotin Standard Solution are added. Free and enzyme-labeled biotin compete for the avidin-binding sites. Any unbound, enzyme-labeled biotin is then removed by a washing step. The detection occurs with the addition of Substrate (urea peroxide) and Chromogen (tetramethylbenzidine). The Conjugate, that is bound to avidin, changes the colorless Chromogen to a blue end product. The addition of the Stop Solution causes a color change from blue to yellow. The measurement is done photometrically at 450 nm (optional: reference wavelength ≥ 600 nm). The resulting absorbance values are inversely proportional to the biotin concentration of the sample.

 

vitamin H ELISA