ECM/Collagens

Collagen Type III, Bovine

bovine type III collagen
Catalog Number: 
8052009
Qty/Size: 
10 mg

bovine collagen type III from MD Bioproducts

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Purified type III bovine collagen.

Overview

Overview: 

Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.

 

Mutations of type III collagen causes Ehlers-Danlos syndrome, EDS IV, which affect arteries, internal organs, joints and skin, and may cause sudden death when the large arteries rupture.

 

Symbols/Related Terms:

 

  • COL3A1
  • Collagen type III, alpha 1
  • Collagen III, alpha-1 polypeptide
  • collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant)
  • EDS4A

 

 

Data/Specifications

Data/Specifications: 

Purity: 

Bovine collagen type III - 90%

Other bovine collagens - 10%

Non-collagen proteins - below 0.5%

 

Form: 10 mg lyophilized, salt-free

 

Source: Bovine (calf ) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type III was purified by using differential salt precipitation.

 

Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.

 

Reconstitution: Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.

 

Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.

Literature/Support

Literature/Support: 

Bovine Type III Collagen Insert (PDF)

Note: inserts are for review only. Please refer to the insert shipped with your product to ensure the most up-to-date revision is being used.

How To Use

How To Use: 

Key Applicatons:

  • Used for rat collagen type I standard. 
  • Antigen for antibody production. 
  • Coating material for cell culture studies. 
  • Collagen microfibrils will activate platelets causing aggregation. 

Note: May not be suitable for 3-D gel formation.