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Highly Purified Human Collagen Type IV for use in surface coating.
Overview
Overview:
Type IV Collagen forms the basal lamina (basement membrane) of epithelia. A meshwork of Type IV collagens provides the filter for the blood capillaries and the glomerli of the kidneys. Type IV collagen, along with laminin, plays an important role in cell adhesion, migration, differentiation, and growth. Degradation of type IV collagen can occur under both physiological and pathological conditions, specifically playing a role during angiogenesis, tissue remodeling, and cancer progression.
Symbols/Related Terms:
- COL4A1
- COL4A2
- COL4A3
- COL4A4
- COL4A5
- COL4A6
Data/Specifications
Data/Specifications:
Concentration: Reconstitute the 5 mg vial with 5 ml of sterile 0.25% acetic acid and mix vigorously until the
contents are completely solubilized.
Purity: 97%, as determined by SDS-PAGE
Source: Human Placenta
Form: Lyophilized, 5 mg/vial
Endotoxin: <0.5 EU/mL (LAL assay)
How To Use
How To Use:
Key Applicatons:
- Surface coating
- 3D gel preparation
Coating Procedure:
- Reconstitute the 5 mg vial with 5 mL of sterile 0.25% acetic acid and mix vigorously until
the contents are completely solubilized. - Dilute the product to desired concentration with 0.25% acetic acid. A typical final coating
concentration may be 10 to 100 μg/cm2. Testing will likely be required to determine optimal
concentrations required for different cell culture systems. - Add appropriate amount of diluted product to culture surface.
- Cover and incubate at room temperature or 37°C for 1 to 2 hours.
- After incubation, aspirate any excess remaining material.
- Rinse coated surface carefully with a sterile balanced salt solution. Avoid scratching
surfaces. - Aspirate remaining material from coated surface.
- Coated culture vessels are now ready to use. The coated culture vessels may be stored
at 2 - 8°C.