cytokine

IL-1 beta ELISA, mouse

mouse IL-1b ELISA
Catalog Number: 
M046052
Qty/Size: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

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For the quantitative determination of IL-1 beta in mouse plasma, serum and cell culture supernate samples.

Overview

Overview: 

Interleukin-beta (IL-1β) has a wide spectrum of inflammatory, metabolic, haemopoietic, and immunological properties. IL-1β plays a significant role in hippocampal synaptic function and is a potential genetic marker as an indicator of gastric cancer risk. High plasma levels of interleukin-1beta (IL-1β) is associated with rheuma- toid and osteoarthritic joint disease, infectious gastroenteritis, neurodegeneration, and breast cancer. High gingival crevicular fluid levels of interleukin-1beta is related to type 2 diabetes.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0.016 - 0.5 ng/mL

Sensitivity:  0.016 ng/mL

Assay Length:   < 5 hours

Literature/Support

Literature/Support: 

How It Works

How It Works: 

Human TNF-α ELISA kit is designed for detection of TNF-α in human plasma, serum or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TNF-α in less than 5 hours. A murine monoclonal antibody specific for human TNF-α has been pre-coated onto a microplate. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

human TNFa ELISA