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PCR Detection of Mycoplasma Contamination in Cell Cultures

The PCR Mycoplasma Test Kit provides a rapid and highly sensitive PCR method for testing your cell cultures for Mycoplasma contamination and contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, Taq polymerase & dNTPs.

Mycoplasma species are often found in research laboratories as contaminants in cell culture. Up to 35% of cell cultures may be contaminated with mycoplasma due to contamination from individuals or contaminated cell culture medium ingredients. Mycoplasma cells are physically small – less than 1 µm, so are difficult to detect with a conventional microscope.

Mycoplasma contamination affects cell functions such as metabolism, morphology, protein synthesis and cell proliferation, leading to unreliable results, lost time and resources. Contamination is persistent and not typically visible by turbidity or microscopy, making it difficult to detect and diagnose. PCR-based assays have the highest sensitivity with minimal preparation time for early detection in a rapid and simple manner, when compared to other methods. Routine screening is essential and traditional methods are time consuming.

Advantages of the PCR Mycoplasma Test Kit:

- Simple and efficient discovery of mycoplasma contamination
- High sensitivity and specificity
- Detects 90 species of Mycoplasma, Acholeplasma, and Spiroplasma
- Detection limit: 10 CFU/mL
- Simple 10 minute protocol

Selection of citations:

Okonska, A., Bühler, S., Rao, V., Ronner, M., Blijlevens, M., van der Meulen-Muileman, I. H., ... & Weder, W. (2020). Functional Genomic Screen in Mesothelioma Reveals that Loss of Function of BRCA1-Associated Protein 1 Induces Chemoresistance to Ribonucleotide Reductase Inhibition. Molecular Cancer Therapeutics, 19(2), 552-563.

Bunch, B. L., Ma, Y., Attwood, K., Amable, L., Luo, W., Morrison, C., ... & Johnson, C. S. (2019). Vitamin D3 enhances the response to cisplatin in bladder cancer through VDR and TA p73 signaling crosstalk. Cancer Medicine.

Nelms, B., Dalomba, N. F., & Lencer, W. (2017). A targeted RNAi screen identifies factors affecting diverse stages of receptor-mediated transcytosis. J Cell Biol, 216(2), 511-525.

Aggarwal, A., Parai, M. K., Shetty, N., Wallis, D., Woolhiser, L., Hastings, C., ... & Wakabayashi, S. (2017). Development of a novel lead that targets M. tuberculosis polyketide synthase 13. Cell, 170(2), 249-259.