MonELISA® Canine IL-17a
MonELISA® Canine IL-17a is an assay using antibodies generated specifically to the entire canine IL-17a protein without any cross-reactivity to other species. This assay provides the most specific results, combined with ease of...
MonELISA® Canine IL-17a is an assay using antibodies generated specifically to the entire canine IL-17a protein without any cross-reactivity to other species. This assay provides the most specific results, combined with ease of use and the uniform MonELISA® assay procedure.
Requires MonELISA® Ancillary Reagent Kit, Catalog Number M036080 (sold separately).
The IL-17 family of cytokines contains six known members as part of the immune system. IL-17a and the structurally close IL-17f play a central role in immune response to pathogens and the development of various inflammatory autoimmune diseases. Psoriasis is one of the most-studied diseases featuring a central role for IL-17a.
This species-specific MonELISA® IL-17a ELISA is a colorimetric based sandwich immunoassay utilizing a polyclonal antibody to the specie’s IL-17a bound on the surface of microwells as the capture antibody and a biotinylated polyclonal antibody to IL-17a as the detection antibody. When antigen is added to the well it is bound by the immobilized antibody. The biotinylated antibody then binds to the antigen forming an antigen-antibody complex. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound. Color development is directly proportional to the antigen concentration in the sample.
Product Insert (PDF) - Informational use only. Please refer to insert included with product.
- Sample Type: canine serum, plasma, cell culture supernatants, tissue homogenates
- Range: 25-1600 pg/mL
- Assay Length: 2 hrs 40 min
- Sample Size: 100 uL
- Sensitivity: < 17.5 pg/mL
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A