Aggrecan Antibody, N-terminal neoepitope FFGV

Catalog Number: 
1042004
Qty/Size: 
100 ug

mouse monoclonal antibody to aggrecan N-terminal Neopepitope FFGV, clone BC14 from MD Bioproducts

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Aggrecan monoclonal antibody to N-terminal neoepitope FFGV (mouse, clone BC14). This fragment is rapidly released from the tissue when MMP catabolism of aggrecan occurs and has been identified in synovial fluid samples from patients with degenerative joint diseases.

Overview

Overview: 

Proteoglycans are categoried depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sullfate) as well as characterized by size.

Aggrecan is a large aggregating proteoglycan of articular cartilage. It is found also in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resiliance during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan bearing aggrecan fragments is one of the earliest events in cartilage destruction.

 

A) Aggrecan monomer showing proteolytic cleavage sites

B) Aggregate showing aggrecan monomers bound to hyaluronic acid

 

Aggrecan

Data/Specifications

Data/Specifications: 

Immunogen: Synthetic peptide: FFGVGGE

 

Clone: BC-14

 

Host: Mouse

 

Myeloma: x63-Ag8.653

 

Isotype: IgG2a

 

Light Chain type: kappa

 

Specificity: Recognizes the N-terminal neoepitope sequence (FFGV....) generated at the “MMP cleavage site” after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration).

 

Cross-reactivity: This antibody cross-reacts with human, Rat, guinea pig, horse, and Pig.

 

Purity: Affinity purified on protein G

 

Form: Liquid, 1 mL/vial

 

Concentration: 0.1 mg/mL

 

Storage: -20° C

Literature/Support

Literature/Support: 

How To Use

How To Use: 

 

  • Western-Blotting - Suggested dilution: 1:100. Detects a a band of approximately 60 - 150 kDa
  • ELISA
  • IHC

 

 

Technical Notes: 

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded

sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01

Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG

of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.