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Aggrecan Antibody, C-terminal neoepitope NITEGE

Catalog Number: 
1042003
Qty/Size: 
100 ug

mouse monoclonal antibody to aggrecan C-terminal neoepitope NITEGE, clone BC-13 from MD Bioproducts

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Aggrecan monoclonal antibody to C-terminal neoepitope NITEGE (mouse, clone BC-13). This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Overview

Overview: 

Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.

Data/Specifications

Data/Specifications: 

Immunogen: NITEGE synthetic peptide conjugate.

 

Clone: BC-13

 

Host: Mouse

 

Myeloma: x63-Ag8.653

 

Isotype: IgG1

 

Light Chain type: kappa

 

Specificity: Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope ...TEGE after cleavage between amino acids ..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).

 

Cross-reactivity: Human, Cow, Dog, Horse, Pig.

 

Purity: Affinity purified on protein G

 

Form: Liquid, 1 mL/vial

 

Concentration: 0.1 mg/mL

 

Storage: -20° C

Literature/Support

Literature/Support: 

How To Use

How To Use: 

Applications

  • Western Blot - Suggested dilution: 1:100. Detects a a band of approximately 60-70 kDa
  • ELISA
  • IHC

 

 

Technical Notes

Samples are usually deglycosylated using 0.01 Units chondroitinase ABC (Sigma), 0.01

Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG

of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry

(1, 2).