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Chondroitinase generated C-4-S & DS Antibody

Catalog Number: 
1042009
Qty/Size: 
100 ug

monoclonal antibody to chondroitinase generated C-4-S & DS, clone 2B6 from MD Bioproducts

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International: +41-44 986 2628

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Chondroitin-4-sulfate (C-4-S) monoclonal antibody that's also specific to dermatan-sulfate (DS) neoepitope (mouse, clone 2B6).

Overview

Overview: 

Monoclonal antibody to delta-unsaturated glucuronic acid adjacent to N-acetylgalactosamine- 4-sulphate in the non-reducing terminal dissacharide “stub” of 4-sulphated chondroitin sulfate that is produced after chondroitinase digestion of chondroitin-4- sulphate glycosaminoglycan chains (chondroitinase ABC or ACII) or dermatan sulphate glycosaminoglycan chains (chondroitinase ABC or chondroitinase B).

Related Terms/Symbols:

  • Chondroitin-4-sulfate
  • C-4-S
  • Dermatan-sulfate

Data/Specifications

Data/Specifications: 

Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).

 

Clone: 2B6

 

Isotype: IgG

 

Host: Mouse

 

Purity: Affinity purified

 

Specificity: Chondroitin-4-sulphate (C-4-S) after treatment with Chondroitinase ABC or ACII. Dermatan sulphate (DS) neoepitope after treatment with Chondroitinase ABC or B.

 

Form: liquid

 

Storage: -20° C

Literature/Support

Literature/Support/Publications: 

Antibody to Chondroitinase-generated C-4-S and DS neoepitope Insert (PDF, 338 KB)


Joint Disease and Aggrecan (blog post)

 

Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393 
Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652 
Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188. 
Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66. 
Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21 
Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334 
Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781 

 

Caterson, B, Christner, JE, Baker, JR, Couchman, JR. (1985) Production and character- ization of monoclonal antibodies directed against connective tissue proteoglycans Fed Proc 44,386-393 

 

Couchman, J.R., Caterson, B., Christner, J.E. and Baker, J.R. (1984). Mapping by Monoclonal Antibody Detection of Glycosaminoglycans in Connective Tissues. Nature 307: 650-652 

 

Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188. 

 

Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66. 

 

Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21 

 

Stefan Milz, Frank Regner, Reinhard Putz, and Michael Benjamin. Expression of a Wide Range of Extracellular Matrix Molecules in the Tendon and Trochlea of the Human Superior Oblique MuscleInvestigative Ophthalmology and Visual Science. 2002;43:1330-1334 

 

Hedlund, H, Hedbom, E, Heinegard, D, Mengarelli-Widholm, S, Reinholt, FP, Svensson, O. (1999) Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage J Biol Chem 274,5777-5781 

How To Use

How To Use: 

Applications

 

  • ELISA
  • Western Blot
  • Immunohistochemistry

 

 

Technical Notes

Pretreatment with Chondroitinase required. 1x10-3 U per 10 mg GAG at 37 °C for 1 hour.