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Collagen Type II Antibody, anti-human

Anti-human Collagen Type II Antibody
Catalog Number: 
203001
Qty/Size: 
0.5 mL

Anti-human Collagen Type II Antibody from MD Bioproducts

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Collagen Type II polyclonal antibody (rabbit anti-human) purified from rabbits injected with type II collagen that was extracted/purified from human cartilage.

 

Overview

Overview: 

Type II collagen (CII) is a fibrillar collagen that is primarily located in cartilage tissue. The major molecular form of collagen in cartilage is type II collagen. Type II collagen is also located in the vitreous humor of the eye, the inner ear, the nose, and the intervertebral discs of the spine. The function of type II collagen is to provide tensile strength to the matrix and give cartilage the ability to resist shearing forces. Mutations of the COL2A1 gene affect the synthesis of type II collagen and cause chondrodysplasias. This is characterized by abnormal cartilage that leads to bone and joint deformities. The degradation of collagen type II is an early indicator of osteoarthritis.

Data/Specifications

Data/Specifications: 

Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 ml di H2O and store aliquots at -20°C.

 

Specificity (% at 1:200 RIA dilution):

Human Collagen, Type II : 100%

Human Collagen, Types I, IV : < 0.1%

Human collagen type III, V, IX, XI : < 1.0%

Human Fibronection : < 0.1%

 

Cross-reactivity:

Human type II procollagen & type II collagen (staining of both matrix & chondrocyte markers is obtained). Significant cross-reactivity has been found by ELISA with bovine Collagen Type II.

 

 

Literature/Support

Literature/Support: 

Anti-Human Collagen Type II Antibody Insert (PDF,  281 KB)

 

References:


Hoffman, J. K., Geraghty, S., & Protzman, N. M. (2015). Articular cartilage repair using marrow stimulation augmented with a viable chondral allograft: 9-month postoperative histological evaluation. Case reports in orthopedics2015.

 

 

Yadav, V., Sun, L., Panilaitis, B., & Kaplan, D. L. (2015). In vitro chondrogenesis with lysozyme susceptible bacterial cellulose as a scaffold. Journal of tissue engineering and regenerative medicine9(12), E276-E288.

 

 

Sutton, T. A., Hato, T., Mai, E., Yoshimoto, M., Kuehl, S., Anderson, M., ... & Dagher, P. C. (2013). p53 is renoprotective after ischemic kidney injury by reducing inflammation. Journal of the American Society of Nephrology24(1), 113-124.

 

 

Dagher, P. C., Mai, E. M., Hato, T., Lee, S. Y., Anderson, M. D., Karozos, S. C., ... & Sutton, T. A. (2011). The p53 inhibitor pifithrin-α can stimulate fibrosis in a rat model of ischemic acute kidney injury. American Journal of Physiology-Renal Physiology302(2), F284-F291.

References/Citations:How the Rabbit Anti-Human Collagen Type II antibody was used:
Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells.
Thorpe SD, et al. Biochem Biophys Res Commun. 2008 Dec; 377(2): 458-62.
The antibody was used for immunohistochemistry analysis (at 3 g/l) of the deposition of collagen type II in cylindrical discs composed of isolated mesenchymal stem cells from porcine femurs. For visualization, a biotin-labelled goat anti-rabbit polyclonal antibody (1 g/l) was used.


How To Use

How To Use: 

For immunostaining of extra or intracellular components in light microscopy and the quantitation of Collagen Type I in biological fluids or in culture supernatants.

 

Applications

  • IFA
  • IHC(p)
  • (SP) RIA
  • ELISA
  • IB/WB

 

Working Dilutions

  • Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen human tissues (liver, skin, placenta...).
  • IHC(p): >1:1000 on fixed, paraffin-embedded human cartilage tissues
  • ELISA: >1:200 (OD >500)