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Collagen Type IV Antibody, anti-mouse

Anti-mouse Collagen Type IV Antibody
Catalog Number: 
203003
Qty/Size: 
0.5 mL

Anti-mouse Collagen Type IV Antibody from MD Bioproducts

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Collagen Type IV polyclonal antibody (rabbit anti-mouse) purified from rabbits injected with type IV collagen that was extracted/purified from mouse tumor tissues.

Overview

Overview: 

Type IV collagen (CIV) is a major structural component of the basal lamina that assembles as a sheetlike network in the underlying extracellular space of all epithelial cells and tubes. The basal lamina separates the epithelia from the underlying connective tissue. There are several different isoforms of type IV collagen, but all are composed in a triple helical structure. The different isoforms are synthesized from six genes: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. Type 1V collagens are considerably more flexible than the fibrillar type collagens. Mutations in genes associated with type IV collagen are associated with Alport and Goodpasture syndrome.

Data/Specifications

Data/Specifications: 

Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 ml di H2O and store aliquots at -20°C.

 

Specificity (% at 1:5000 RIA dilution):

Mouse Collagen, Type IV : 100%

Mouse Collagen, Types I, II, III : < 0.1%

Human Collagen Type  IV, V: <0.1%

Mouse fibronectin: <0.1%

Mouse laminin: <0.1%

 

 

Literature/Support

Literature/Support: 

How To Use

How To Use: 

For immunostaining of extra or intracellular components in light microscopy and the quantitation of Collagen Type II in biological fluids or in culture supernatants.

 

Applications

  • IFA
  • RIA
  • ELISA
  • WB

 

Working Dilutions

  • Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:40 on mouse articular cartilage tissue