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Keratan Sulfate Antibody

Monoclonal Antibody to Keratan Sulfate, clone 5D4
Catalog Number: 
1042010
Qty/Size: 
100 ug

Monoclonal Antibody to keratan sulfate, clone 5D4 from MD Bioproducts

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Keratan sulfate (KS) monoclonal antibody (mouse, clone 5D4) used to detect KS type I epitopes and KS type II epitopes.

Overview

Overview: 

Keratan Sulfate (KS) is a proteoglycan found in cornea (KS type I), cartilage and bone (KS type II). It’s KS chains are attached to extracellular matrix proteins or core proteins, which include lumican, keratocan, mimecan, fibromodulin, PRELP, osteoaheran and aggrecan. In the joints, keratan sulfate acts as a cushion to protect against mechanical shock. This monoclonal antibody has been raised against sulfated oligosaccharide present in corneal (KS I) and skeletal keratan sulfate (KS II).


Related Terms/Symbols:

  • Keratan sulfate proteoglycans
  • KS
  • KS Type I
  • KS Type II

Data/Specifications

Data/Specifications: 

Immunogen: Keratan sulfate

 

Clone: 5D4

 

Isotype: IgG

 

Host: Mouse

 

Specificity: Highly sulfated keratan sulfate glycosaminoglycan chaines in both Type I and Type II KS.

 

Purity: Affinity purified

 

Form: Liquid

 

Storage: -20° C

Literature/Support

Literature/Support/Publications: 

Keratocan Sulfate Antibody Insert (PDF)


Joint Disease and Aggrecan (blog post)

 

Caterson B, Christner JE & Baker JR (1983). Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibod- ies to cartilage proteoglycan. J Biol Chem. 258(14): 8848-54. 
Thonar EJ, Lenz ME, Klintworth GK, Caterson B, Pachman LM, Glickman P, Katz R, Huff J & Kuettner KE (1985). Quantification of keratan sulfate in blood as a marker of cartilage catabolism. Arthritis & Rheum. 28(12): 1367-76. 
Mehmet H, Scudder P, Tang PW, Hounsell EF, Caterson B & Feizi T (1986). The anti- genic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series. Eur J Biochem. 157(2): 385-91. 
Funderburgh JL, Caterson B & Conrad GW (1987). Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan. J Biol Chem. 262(24):11634-40. 
Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188. 
Young RD, Akama TO, Liskova P, Ebenezer ND, Allan B, Kerr B, Caterson B, Fukuda MN, Quantock AJ (2007a). Differential immunogold localisation of sulphated and unsul- phated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies. Histochem Cell Biol. 127(1):115-20 
Young RD, Gealy EC, Liles M, Caterson B, Ralphs JR & Quantock AJ (2007b). Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimen- tary lamellae in the developing avian cornea. Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3083-8. 
Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66. 
Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21 

Caterson B, Christner JE & Baker JR (1983). Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibod- ies to cartilage proteoglycan. J Biol Chem. 258(14): 8848-54. 

 

Thonar EJ, Lenz ME, Klintworth GK, Caterson B, Pachman LM, Glickman P, Katz R, Huff J & Kuettner KE (1985). Quantification of keratan sulfate in blood as a marker of cartilage catabolism. Arthritis & Rheum. 28(12): 1367-76. 

 

Mehmet H, Scudder P, Tang PW, Hounsell EF, Caterson B & Feizi T (1986). The anti- genic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series. Eur J Biochem. 157(2): 385-91. 

 

Funderburgh JL, Caterson B & Conrad GW (1987). Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan. J Biol Chem. 262(24):11634-40. 

 

Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B & Dent CM (2000). Catabolism of aggrecan, decorin and biglycan in tendon. Biochem J. 350: 181-188. 

 

Young RD, Akama TO, Liskova P, Ebenezer ND, Allan B, Kerr B, Caterson B, Fukuda MN, Quantock AJ (2007a). Differential immunogold localisation of sulphated and unsul- phated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies. Histochem Cell Biol. 127(1):115-20 

 

Young RD, Gealy EC, Liles M, Caterson B, Ralphs JR & Quantock AJ (2007b). Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimen- tary lamellae in the developing avian cornea. Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3083-8. 

 

Hayes AJ, Hall A, Brown L, Tubo R & Caterson B (2007). Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem. 55(8):853-66. 

 

Hayes AJ, Hughes CE & Caterson B (2008). Antibodies and immunohistochemisrty in extracellular matrix research. Methods 45: 10-21 

How To Use

How To Use: 

 

Applications

ELISA

Western Blot

Immunohistochemistry

 

Technical Notes

No enzyme pretreatment required.