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Lumican, Antibody

Monoclonal Antibody to Lumican, clone Lum-1
Catalog Number: 
1042007
Qty/Size: 
100 ug

Monoclonal Antibody to Lumican, clone Lum-1 from MD Bioproducts

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Lumican (LUM) monoclonal antibody (mouse, clone Lum-1) to detect a protein epitope in lumican.

Overview

Overview: 

Lumican (LUM) is the major keratan sulfate proteoglycan of the cornea and it binds collagen fibrils. In noncorneal tissues, lumican is present as a low or nonsulfated glycoprotein and has been found in the aorta, cartilage, liver, skeletal muscle, kidney, pancreas, brain, placenta and lung. Complications of the cornea have been confirmed using lumican-null mice. These mice exhibit corneal opacity, skin fragility, and impaired collagen fibrillogenesis. More recent studies show lumican has an integral role in cell migration, adhesion and proliferation. The expression of lumican has also been examined in breast, pancreatic and colorectal cancer. The affect of lumican depends on the type of cancer as it has been shown to have both a positive and negative affect on tumor growth.


Related Terms/Symbols

  • LUM
  • LDC
  • SLRR2D
  • P51884
  • Lumican proteoglycan
  • major keratan sulfate proteoglycan

 

 

Data/Specifications

Data/Specifications: 

 

Clone: Lum-1

 

Host: Mouse

 

Isotype: IgM

 

Purity: Affinity purified on thiophilic column

 

Form: Liquid, 1 mL/vial

 

Concentration: 0.1 mg/mL

 

Storage: -20°C.

 

Specificity: This Lumican antibody recognises a protein epitope in lumican derived from human, bovine, porcine & mouse tissues.

Literature/Support

Literature/Support/Publications: 

Lumican Antibody Insert (PDF, 311 KB)


Keratocan and lumican play an essential role in corneal transparency of the eye (Blog post - April 19, 2010)

 

Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degener- ate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Melrose J, Fuller ES, Roughley PJ, Smith MM, Kerr B, Hughes CE, Caterson B, Little CB. (2008) Arthritis Res Ther. 10(4):R79. 
Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. Hayashida Y, Akama TO, Beecher N, Lewis P, Young RD, Meek KM, Kerr B, Hughes CE, Caterson B, Tanigami A, Nakayama J, Fukada MN, Tano Y, Nishida K, Quantock AJ. (2006) Proc Natl Acad Sci U S A. 5;103(36):13333-8. 

Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degener- ate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Melrose J, Fuller ES, Roughley PJ, Smith MM, Kerr B, Hughes CE, Caterson B, Little CB. (2008) Arthritis Res Ther. 10(4):R79. 

 

Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. Hayashida Y, Akama TO, Beecher N, Lewis P, Young RD, Meek KM, Kerr B, Hughes CE, Caterson B, Tanigami A, Nakayama J, Fukada MN, Tano Y, Nishida K, Quantock AJ. (2006) Proc Natl Acad Sci U S A. 5;103(36):13333-8. 

How To Use

How To Use: 

 

Applications

 

  • Western Blot: recommended dilution 0.2 mg/mL
  • IHC

 

 

Technical Notes

This epitope is best recognized after keratanase and keratanase II treatment/digestion

of section (IHC) or tissue extracts (for Western analyses). In some IHC tissue analyses,

chondroitinase treatment may also be used, as this second enzyme treatment facilitates

antibody penetration into the tissue.

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