ST2L Human, Monoclonal Antibody
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Human ST2L monoclonal antibody (clone B4E6) for the identification and purification of human type 2 (Th2) lymphocyte subsets.
Overview
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.
Data/Specifications
Clone: B4E6
IgG Subclass: IgG1
Form: The antibody is supplied in PBS and purified over protein G-sepharose from concentrated tissue culture supernatant.
Specificity: This clone recognizes hST2L specifically on the cell surface of human type 2 (Th2, Tc2, NK2 and NKT2) but not type 1 lymphocytes.
Literature/Support
ST2L Monoclonal Antibody (product insert)
Understanding The Four Isoforms of ST2 (blog post)
Van der Jeught, K., Sun, Y., Fang, Y., Zhou, Z., Jiang, H., Yu, T., ... & Eyvani, H. (2020). ST2 as checkpoint target for colorectal cancer immunotherapy. JCI insight, 5(9).
Takaki-Kuwahara, A., Arinobu, Y., Miyawaki, K., Yamada, H., Tsuzuki, H., Irino, K., ... & Tsukamoto, H. (2019). CCR6+ group 3 innate lymphoid cells accumulate in inflamed joints in rheumatoid arthritis and produce Th17 cytokines. Arthritis Research & Therapy, 21(1), 1-9.
Kim, J., Chang, Y., Bae, B., Sohn, K. H., Cho, S. H., Chung, D. H., ... & Kim, H. Y. (2018). Innate immune crosstalk in asthmatic airways: innate lymphoid cells coordinate the polarization of lung macrophage. Journal of Allergy and Clinical Immunology.
Cai, T., Qiu, J., Ji, Y., Li, W., Ding, Z., Suo, C., ... & Guo, X. (2018). IL-17–producing ST2+ group 2 innate lymphoid cells play a pathogenic role in lung inflammation. Journal of Allergy and Clinical Immunology.
Huang, T., Hazen, M., Shang, Y., Zhou, M., Wu, X., Yan, D., ... & Shi, Y. (2016). Depletion of major pathogenic cells in asthma by targeting CRTh2. JCI Insight, 1(7).
Monticelli, L. A., Buck, M. D., Flamar, A. L., Saenz, S. A., Wojno, E. D. T., Yudanin, N. A., ... & Shah, H. (2016). Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation. Nature Immunology, 17(6), 656
Sattler, S., Ling, G. S., Xu, D., Hussaarts, L., Romaine, A., Zhao, H., ... & Lau, Y. L. (2014). IL-10-producing regulatory B cells induced by IL-33 (BregIL-33) effectively attenuate mucosal inflammatory responses in the gut. Journal of autoimmunity, 50, 107-122.
Yang, Q., Li, G., Zhu, Y., Liu, L., Chen, E., Turnquist, H., ... & Lu, B. (2011). IL‐33 synergizes with TCR and IL‐12 signaling to promote the effector function of CD8+ T cells. European journal of immunology, 41(11), 3351-3360.
Clark, R. A., Chong, B., Mirchandani, N., Brinster, N. K., Yamanaka, K. I., Dowgiert, R. K., & Kupper, T. S. (2006). The vast majority of CLA+ T cells are resident in normal skin. The Journal of Immunology, 176(7), 4431-4439
| References/Citations: | The ST2L Antibody was used for: |
Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells? Chan WL, et al. Cellular Immun (2005) 233:109-114
| Application of ST2L and IL18R markers in absdominal aortic aneurysms |
| Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. | Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies. |
| Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells Chan, W.L. et al., Circ Res (2005) 96:675-683 | Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA) |
| NKT cell subsets in infection and inflammation Chan WL, et al. Immun Lett (2003) 85:159-163 | Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis |
| Regulation of ST2L expression on T helper (Th) type 2 cells Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985 | Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells |
| Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44. | ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention. |
How To Use
Applications
- Flow cytometry
- FACS
- cell sorting
- immunohistochemistry with hybridoma culture supernatant