T1/ST2 (IL-33 R) Monoclonal Antibody

Monoclonal Antibody to mouse T1/ST2 (IL-33 R), clone DJ8
Catalog Number: 
101001
Qty/Size: 
0.5 mL

Monoclonal Antibody to mouse T1/ST2 (IL-33 R), clone DJ8 from MD Bioproducts

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Mouse T1/ST2 (IL-33 R) monoclonal antibody (clone DJ8) for the identification and purification of murine T helper 2 (Th2) cells and all forms of murine mast cells.

Overview

Overview: 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for murine Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.

Data/Specifications

Data/Specifications: 

Rat anti-Mouse T1/ST2 purified mAb

Clone: DJ8

 

IgG Subclass: IgG1 (light chain not isotyped)

 

Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.

 

Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine stimulated murine fibroblasts.

 

Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.

Literature/Support

Literature/Support: 

T1/ST2 Monoclonal Antibody (PDF, product insert)

 

Understanding The Four Isoforms of ST2 (blog post)

 

Bando JK, Liang HE, Locksley RM (2015) Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol. 2015 Feb;16(2):153-60

 

Filbey KJ, Grainger JR, Smith KA, Boon L, van Rooijen N, Harcus Y, Jenkins S, Hewitson JP, Maizels RM (2014) Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection. Immunol Cell Biol. 2014 May-Jun;92(5):436-48.

 

Biethahn, K., Orinska, Z., Vigorito, E., Goyeneche‐Patino, D. A., Mirghomizadeh, F., Föger, N., & Bulfone‐Paus, S. (2014). miRNA‐155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model. Allergy, 69(6), 752-762.

 

Connor, L. M., Tang, S. C., Camberis, M., Le Gros, G., & Ronchese, F. (2014). Helminth-conditioned dendritic cells prime CD4+ T cells to IL-4 production in vivo. The Journal of Immunology, 193(6), 2709-2717.

 

Beale, J., Jayaraman, A., Jackson, D. J., Macintyre, J. D., Edwards, M. R., Walton, R. P., ... & Bartlett, N. W. (2014). Rhinovirus-induced IL-25 in asthma exacerbation drives type 2 immunity and allergic pulmonary inflammation. Science translational medicine, 6(256), 256ra134-256ra134.

 

Matta, B. M., Lott, J. M., Mathews, L. R., Liu, Q., Rosborough, B. R., Blazar, B. R., & Turnquist, H. R. (2014). IL-33 is an unconventional alarmin that stimulates IL-2 secretion by dendritic cells to selectively expand IL-33R/ST2+ regulatory T cells. The Journal of Immunology, 193(8), 4010-4020.

 

Furusawa, J. I., Moro, K., Motomura, Y., Okamoto, K., Zhu, J., Takayanagi, H., ... & Koyasu, S. (2013). Critical role of p38 and GATA3 in natural helper cell function. The Journal of Immunology, 191(4), 1818-1826.

 

Imai, Y., Yasuda, K., Sakaguchi, Y., Haneda, T., Mizutani, H., Yoshimoto, T., ... & Yamanishi, K. (2013). Skin-specific expression of IL-33 activates group 2 innate lymphoid cells and elicits atopic dermatitis-like inflammation in mice. Proceedings of the National Academy of Sciences, 110(34), 13921-13926.

 

Lipsky, B. P., Toy, D. Y., Swart, D. A., Smithgall, M. D., & Smith, D. (2012). Deletion of the ST2 proximal promoter disrupts fibroblast‐specific expression but does not reduce the amount of soluble ST2 in circulation. European journal of immunology, 42(7), 1863-1869.

 

Bartemes, K. R., Iijima, K., Kobayashi, T., Kephart, G. M., McKenzie, A. N., & Kita, H. (2012). IL-33–Responsive Lineage− CD25+ CD44hi Lymphoid Cells Mediate Innate Type 2 Immunity and Allergic Inflammation in the Lungs. The Journal of Immunology, 188(3), 1503-1513.

 

Seol, D., McCabe, D. J., Choe, H., Zheng, H., Yu, Y., Jang, K., & Martin, J. A. (2012). Chondrogenic progenitor cells respond to cartilage injury. Arthritis & Rheumatism, 64(11), 3626-3637.

 

Doherty, T. A., Khorram, N., Chang, J. E., Kim, H. K., Rosenthal, P., Croft, M., & Broide, D. H. (2012). STAT6 regulates natural helper cell proliferation during lung inflammation initiated by Alternaria. American Journal of Physiology-Lung Cellular and Molecular Physiology, 303(7), L577-L588.

 

Turnquist, H. R., Zhao, Z., Rosborough, B. R., Liu, Q., Castellaneta, A., Isse, K., ... & Thomson, A. W. (2011). IL-33 expands suppressive CD11b+ Gr-1int and regulatory T cells, including ST2L+ Foxp3+ cells, and mediates regulatory T cell-dependent promotion of cardiac allograft survival. The Journal of Immunology, 187(9), 4598-4610.

 

Anthony, R. M., Kobayashi, T., Wermeling, F., & Ravetch, J. V. (2011). Intravenous gammaglobulin suppresses inflammation through a novel TH2 pathway. Nature, 475(7354), 110-113.

 

Navarro, S., Cossalter, G., Chiavaroli, C., Kanda, A., Fleury, S., Lazzari, A., ... & Julia, V. (2011). The oral administration of bacterial extracts prevents asthma via the recruitment of regulatory T cells to the airways. Mucosal immunology, 4(1), 53-65.

 

Ohno, T., Oboki, K., Morita, H., Kajiwara, N., Arae, K., Tanaka, S., & Nakae, S. (2011). Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. PLoS One, 6(4), e18404.

 

Föger, N., Jenckel, A., Orinska, Z., Lee, K. H., Chan, A. C., & Bulfone-Paus, S. (2011). Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and Coronin1b. The Journal of experimental medicine, 208(9), 1777-1787.

 

Schulze, J., Bickert, T., Beil, F. T., Zaiss, M. M., Albers, J., Wintges, K., & Schinke, T. (2011). Interleukin‐33 is expressed in differentiated osteoblasts and blocks osteoclast formation from bone marrow precursor cells. Journal of Bone and Mineral Research, 26(4), 704-717.

 

Rankin, A. L., Mumm, J. B., Murphy, E., Turner, S., Yu, N., McClanahan, T. K., ... & Pflanz, S. (2010). IL-33 induces IL-13–dependent cutaneous fibrosis. The journal of immunology, 184(3), 1526-1535.

 

Cunnusamy, K., Chen, P. W., & Niederkorn, J. Y. (2010). IL-17 promotes immune privilege of corneal allografts. The Journal of Immunology, 185(8), 4651-4658.

 

Alves-Filho, J. C., Sônego, F., Souto, F. O., Freitas, A., Verri Jr, W. A., Auxiliadora-Martins, M., & Liew, F. Y. (2010). Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of infection. Nature medicine, 16(6), 708-712.

 

Moro, K., Yamada, T., Tanabe, M., Takeuchi, T., Ikawa, T., Kawamoto, H., & Koyasu, S. (2010). Innate production of TH2 cytokines by adipose tissue-associated c-Kit+ Sca-1+ lymphoid cells. Nature, 463(7280), 540-544.

 

References/Citations:How the T1/ST2, monoclonal antibody was used:
IL-33 induces IL-13-dependent cutaneous fibrosis.
Rankin A, et al. Journ. of Immunol., 2010; 184(3):1526-35.
Flow cytometry analysis of bone marrow-derived eosinophils isolated from C57BL/6 and genetically modified mice.

IL-33 Enhances Lipopolysaccharide-Induced Inflammatory Cytokine Production from Mouse Macrophages by Regulating Lipopolysaccharide Receptor Complex
Quentin Espinassous et al., J. Immunol., Jul 2009; 183: 1446 - 1455.

As a blocking agent in cellular assays of peritoneal macrophages(10^6) isolated from mice. The antibody was also used for immunoprecipitation.
Interleukin-1 receptor-related protein ST2 suppresses the initial stage of bleomycin-induced lung injury
N. Mato et al., Eur. Respir. J., Jun 2009; 33: 1415 - 1428.

Constructed an ELISA system for determining the concentration of ST2 in mouse plasma samples, bronchoalveolar fluid and lung homogenates.

IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
Chackerian A, et al. Journ. of Immunol., 2007; 179(4):2551-5.

Blocking agent for IL-33 and IL-33R pathway analysis.

IL-17 Promotes Immune Privilege of Corneal Allografts
Khrishen Cunnusamy, Peter W. Chen, and Jerry Y. Niederkorn
J. Immunol., Oct 2010; 185: 4651 - 4658.
Sections (4 µm) of paraffin-embedded tissue were labeled with mAb against T1/ST2 (DJ8; MD Biosciences) to detect Th2 cells

Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells?  Chan WL, et al. Cellular Immun (2005) 233:109-114

Application of ST2L and IL18R markers in absdominal aortic aneurysms
Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells  Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. 
Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies.
Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells  Chan, W.L. et al., Circ Res (2005) 96:675-683
Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA)
NKT cell subsets in infection and inflammation  Chan WL, et al. Immun Lett (2003) 85:159-163
Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis
Regulation of ST2L expression on T helper (Th) type 2 cells  Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985
Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells
Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes  Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention.

 

 

 

 

How To Use

How To Use: 

 

Applications

 

  • Flow cytometry
  • immunoprecipitation