T1/ST2 (IL-33 R) Monoclonal Antibody, azide-free
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Mouse T1/ST2 (IL-33 R) monoclonal antibody (clone DJ8) for the identification and purification of murine T helper 2 (Th2) cells and all forms of murine mast cells.
Overview
T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.
Data/Specifications
Rat anti-Mouse T1/ST2 purified mAb, azide-free
Clone: DJ8
IgG Subclass: IgG1 (light chain not isotyped)
Form: The antibody is supplied in PBS and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.
Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine stimulated murine fibroblasts.
Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.
Literature/Support
T1/ST2 Monoclonal Antibody, azide-free (PDF, product insert)
Understanding The Four Isoforms of ST2 (blog post)
| References/Citations: | How the T1/ST2, monoclonal antibody was used: |
| IL-33 induces IL-13-dependent cutaneous fibrosis. Rankin A, et al. Journ. of Immunol., 2010; 184(3):1526-35. | Flow cytometry analysis of bone marrow-derived eosinophils isolated from C57BL/6 and genetically modified mice. |
IL-33 Enhances Lipopolysaccharide-Induced Inflammatory Cytokine Production from Mouse Macrophages by Regulating Lipopolysaccharide Receptor Complex | As a blocking agent in cellular assays of peritoneal macrophages(10^6) isolated from mice. The antibody was also used for immunoprecipitation. |
| Interleukin-1 receptor-related protein ST2 suppresses the initial stage of bleomycin-induced lung injury N. Mato et al., Eur. Respir. J., Jun 2009; 33: 1415 - 1428. | Constructed an ELISA system for determining the concentration of ST2 in mouse plasma samples, bronchoalveolar fluid and lung homogenates. |
| IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. Chackerian A, et al. Journ. of Immunol., 2007; 179(4):2551-5. | Blocking agent for IL-33 and IL-33R pathway analysis. |
| IL-17 Promotes Immune Privilege of Corneal Allografts Khrishen Cunnusamy, Peter W. Chen, and Jerry Y. Niederkorn J. Immunol., Oct 2010; 185: 4651 - 4658. | Sections (4 µm) of paraffin-embedded tissue were labeled with mAb against T1/ST2 (DJ8; MD Biosciences) to detect Th2 cells |
Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells? Chan WL, et al. Cellular Immun (2005) 233:109-114 | Application of ST2L and IL18R markers in absdominal aortic aneurysms |
| Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. | Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies. |
| Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells Chan, W.L. et al., Circ Res (2005) 96:675-683 | Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA) |
| NKT cell subsets in infection and inflammation Chan WL, et al. Immun Lett (2003) 85:159-163 | Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis |
| Regulation of ST2L expression on T helper (Th) type 2 cells Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985 | Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells |
| Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44. | ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention. |
How To Use
Applications
- Flow cytometry
- immunoprecipitation