T1/ST2 (IL-33 R) Mouse, Monoclonal Antibody, Biotinylated

Catalog Number: 
101001B
Qty/Size: 
0.5 mL

Monoclonal Antibody to mouse T1/ST2 (IL-33 R), clone DJ8 from MD Bioproducts

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International: +41-44 986 2628

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Mouse T1/ST2 (IL-33 R) biotinylated monoclonal antibody (clone DJ8) for the identification and purification of Th2 cells and all forms of murine mast cells. 

Overview

Overview: 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.

Data/Specifications

Data/Specifications: 

Rat anti-Mouse T1/ST2 biotinylated mAb

Clone: DJ8

 

Ig Subclass: IgG1 (light chain not isotyped)

 

Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.

 

Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine-stimulated murine fibroblasts.

 

Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.

Literature/Support

Literature/Support: 

T1/ST2 Monoclonal Antibody, Biotinylated (PDF, product insert)

 

Understanding The Four Isoforms of ST2 (blog post)

 

Jin, R. M., Warunek, J., & Wohlfert, E. A. (2018).Therapeutic Administration of IL-10 and Amphiregulin Alleviates Chronic Skeletal Muscle Inflammation and Damage Induced by Infection. ImmunoHorizons2(5), 142-154.

 

Cayrol, C., Duval, A., Schmitt, P., Roga, S., Camus, M., Stella, A., ... & Girard, J. P. (2018). Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33. Nature immunology19(4), 375.

 

Schneider, C., O’Leary, C. E., von Moltke, J., Liang, H. E., Ang, Q. Y., Turnbaugh, P. J., ... & Locksley, R. M. (2018). A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling. Cell.

 

Laman, J. D., Kooistra, S. M., & Clausen, B. E. (2017). Reproducibility issues: avoiding pitfalls in animal inflammation models. In Inflammation (pp. 1-17). Humana Press, New York, NY.

 

Tsuzuki, H., Arinobu, Y., Miyawaki, K., Takaki, A., Ota, S. I., Ota, Y., ... & Niiro, H. (2017). Functional interleukin‐33 receptors are expressed in early progenitor stages of allergy‐related granulocytes. Immunology150(1), 64-73.

 

Chen, C. C., Kobayashi, T., Iijima, K., Hsu, F. C., & Kita, H. (2017). IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs. Journal of Allergy and Clinical Immunology140(5), 1351-1363.

 

Dominguez, D., Ye, C., Geng, Z., Chen, S., Fan, J., Qin, L., ... & Fang, D. (2017). Exogenous IL-33 restores dendritic cell activation and maturation in established cancer. The Journal of Immunology198(3), 1365-1375.

 

Zarnegar, B., Mendez-Enriquez, E., Westin, A., Söderberg, C., Dahlin, J. S., Grönvik, K. O., & Hallgren, J. (2017). Influenza infection in mice induces accumulation of lung mast cells through the recruitment and maturation of mast cell progenitors. Frontiers in immunology8, 310.

 

Morikawa, T., Fukuoka, A., Matsushita, K., Yasuda, K., Iwasaki, N., Akasaki, S., ... & Yoshimoto, T. (2017). Activation of group 2 innate lymphoid cells exacerbates and confers corticosteroid resistance to mouse nasal type 2 inflammation. International immunology29(5), 221-233.

 

Dullaers, M., Schuijs, M. J., Willart, M., Fierens, K., Van Moorleghem, J., Hammad, H., & Lambrecht, B. N. (2017). House dust mite–driven asthma and allergen-specific T cells depend on B cells when the amount of inhaled allergen is limiting. Journal of Allergy and Clinical Immunology140(1), 76-88.

 

He, Z., Chen, L., Souto, F. O., Canasto-Chibuque, C., Bongers, G., Deshpande, M., ... & Lira, S. A. (2017). Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc Min/+ mice. Scientific reports7(1), 5520.

 

Mowel, W. K., McCright, S. J., Kotzin, J. J., Collet, M. A., Uyar, A., Chen, X., ... & Villarino, A. (2017). Group 1 innate lymphoid cell lineage identity is determined by a cis-regulatory element marked by a long non-coding RNA. Immunity47(3), 435-449.

 

Minutti, C. M., Drube, S., Blair, N., Schwartz, C., McCrae, J. C., McKenzie, A. N., ... & Sijts, A. J. (2017). Epidermal growth factor receptor expression licenses type-2 helper T cells to function in a T cell receptor-independent fashion. Immunity47(4), 710-722.

 

Yang, Q., Moyar, Q. G., Kokalari, B., Redai, I. G., Wang, X., Kemeny, D. M., ... & Haczku, A. (2016). Group 2 innate lymphoid cells mediate ozone-induced airway inflammation and hyperresponsiveness in mice. Journal of Allergy and Clinical Immunology137(2), 571-578.

 

Rak, G. D., Osborne, L. C., Siracusa, M. C., Kim, B. S., Wang, K., Bayat, A., ... & Volk, S. W. (2016). IL-33-dependent group 2 innate lymphoid cells promote cutaneous wound healing. Journal of Investigative Dermatology136(2), 487-496.

 

Biton, J., Athari, S. K., Thiolat, A., Santinon, F., Lemeiter, D., Hervé, R., ... & Girard, J. P. (2016). In vivo expansion of activated Foxp3+ regulatory T cells and establishment of a type 2 immune response upon IL-33 treatment protect against experimental arthritis. The Journal of Immunology, 1502124.

 

Gordon, E. D., Simpson, L. J., Rios, C. L., Ringel, L., Lachowicz-Scroggins, M. E., Peters, M. C., ... & Yuan, S. (2016). Alternative splicing of interleukin-33 and type 2 inflammation in asthma. Proceedings of the National Academy of Sciences113(31), 8765-8770.

 

Tsuzuki, H., Arinobu, Y., Miyawaki, K., Takaki, A., Ota, S. I., Ota, Y., ... & Niiro, H. (2017). Functional interleukin‐33 receptors are expressed in early progenitor stages of allergy‐related granulocytes. Immunology150(1), 64-73.

 

Yoon, J., Leyva-Castillo, J. M., Wang, G., Galand, C., Oyoshi, M. K., Kumar, L., ... & Kuchroo, V. K. (2016). IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. Journal of Experimental Medicine213(10), 2147-2166.

 

Jackson-Jones, L. H., Duncan, S. M., Magalhaes, M. S., Campbell, S. M., Maizels, R. M., McSorley, H. J., ... & Bénézech, C. (2016). Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nature communications7, 12651.

 

Valladao, A. C., Frevert, C. W., Koch, L. K., Campbell, D. J., & Ziegler, S. F. (2016). STAT6 regulates the development of eosinophilic versus neutrophilic asthma in response to Alternaria alternata. The Journal of Immunology, 1600007.

 

Van Dyken, S. J., Nussbaum, J. C., Lee, J., Molofsky, A. B., Liang, H. E., Pollack, J. L., ... & Erle, D. J. (2016). A tissue checkpoint regulates type 2 immunity. Nature immunology17(12), 1381.

 

Paclik, D., Stehle, C., Lahmann, A., Hutloff, A., & Romagnani, C. (2015). ICOS regulates the pool of group 2 innate lymphoid cells under homeostatic and inflammatory conditions in mice. European journal of immunology45(10), 2766-2772.

 

Walker, J. A., Oliphant, C. J., Englezakis, A., Yu, Y., Clare, S., Rodewald, H. R., ... & McKenzie, A. N. (2015). Bcl11b is essential for group 2 innate lymphoid cell development. Journal of Experimental Medicine212(6), 875-882.

 

Zhang, J., Ramadan, A. M., Griesenauer, B., Li, W., Turner, M. J., Liu, C., ... & Paczesny, S. (2015). ST2 blockade reduces sST2-producing T cells while maintaining protective mST2-expressing T cells during graft-versus-host disease. Science translational medicine7(308), 308ra160-308ra160.

 

Cui, Y., Dahlin, J. S., Feinstein, R., Bankova, L. G., Xing, W., Shin, K., ... & Hallgren, J. (2014). Mouse mast cell protease-6 and MHC are involved in the development of experimental asthma. The Journal of Immunology, 193(10), 4783-4789.

 

Kim, B. S., Wang, K., Siracusa, M. C., Saenz, S. A., Brestoff, J. R., Monticelli, L. A., ... & Artis, D. (2014). Basophils promote innate lymphoid cell responses in inflamed skin. The Journal of Immunology, 193(7), 3717-3725

 

Kato, Y., Akasaki, S., Muto-Haenuki, Y., Fujieda, S., Matsushita, K., & Yoshimoto, T. (2014). Nasal sensitization with ragweed pollen induces local-allergic-rhinitis-like symptoms in mice.

 

Komai‐Koma, M., Li, D., Wang, E., Vaughan, D., & Xu, D. (2014). Anti‐Toll‐like receptor 2 and 4 antibodies suppress inflammatory response in mice. Immunology, 143(3), 354-362.

 

Heger, K., Seidler, B., Vahl, J. C., Schwartz, C., Kober, M., Klein, S., ... & Schmidt‐Supprian, M. (2014). CreERT2 expression from within the c‐Kit gene locus allows efficient inducible gene targeting in and ablation of mast cells. European journal of immunology, 44(1), 296-306.

 

Spooner, C. J., Lesch, J., Yan, D., Khan, A. A., Abbas, A., Ramirez-Carrozzi, V., ... & Singh, H. (2013). Specification of type 2 innate lymphocytes by the transcriptional determinant Gfi1. Nature immunology, 14(12), 1229-1236.

 

Nussbaum, J. C., Van Dyken, S. J., von Moltke, J., Cheng, L. E., Mohapatra, A., Molofsky, A. B., ... & Locksley, R. M. (2013). Type 2 innate lymphoid cells control eosinophil homeostasis. Nature, 502(7470), 245-248.

 

Saenz, S. A., Siracusa, M. C., Monticelli, L. A., Ziegler, C. G., Kim, B. S., Brestoff, J. R., ... & Artis, D. (2013). IL-25 simultaneously elicits distinct populations of innate lymphoid cells and multipotent progenitor type 2 (MPPtype2) cells. The Journal of experimental medicine, 210(9), 1823-1837.

 

Kamijo, S., Takeda, H., Tokura, T., Suzuki, M., Inui, K., Hara, M., ... & Takai, T. (2013). IL-33–Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen–Induced Allergic Airway Inflammation. The Journal of Immunology, 190(9), 4489-4499.

 

Molofsky, A. B., Nussbaum, J. C., Liang, H. E., Van Dyken, S. J., Cheng, L. E., Mohapatra, A., ... & Locksley, R. M. (2013). Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages. The Journal of experimental medicine, 210(3), 535-549.

 

Nussbaum, J. C., Van Dyken, S. J., von Moltke, J., Cheng, L. E., Mohapatra, A., Molofsky, A. B., ... & Locksley, R. M. (2013). Type 2 innate lymphoid cells control eosinophil homeostasis. Nature, 502(7470), 245-248.

 

Wong, S. H., Walker, J. A., Jolin, H. E., Drynan, L. F., Hams, E., Camelo, A., ... & McKenzie, A. N. (2012). Transcription factor ROR [alpha] is critical for nuocyte development. Nature immunology, 13(3), 229-236.

 

Chang, Y. J., Kim, H. Y., Albacker, L. A., Baumgarth, N., McKenzie, A. N., Smith, D. E., ... & Umetsu, D. T. (2011). Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity. Nature immunology, 12(7), 631-638.

 

Saenz, S. A., Siracusa, M. C., Perrigoue, J. G., Spencer, S. P., Urban Jr, J. F., Tocker, J. E., ... & Artis, D. (2010). IL25 elicits a multipotent progenitor cell population that promotes TH2 cytokine responses. Nature, 464(7293), 1362-1366.

 

References/Citations:

How the T1/ST2 Biotinylated antibody was used:
Cutting Edge: Atopy Promotes Th2 Responses to Alloantigens and Increases the Incidence and Tempo of Corneal Allograft Rejection
Clay Beauregard et al., J. Immunol., Jun 2005; 174: 6577 - 6581.

Immunohistochemistry analysis to detect the presence of Th2 cells in four-micrometer cross sections of the eye. Eyes were obtained from C57/BL/6 and BALB/c mice.

Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells?  Chan WL, et al. Cellular Immun (2005) 233:109-114

Application of ST2L and IL18R markers in absdominal aortic aneurysms
Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells  Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. 
Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies.
Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells  Chan, W.L. et al., Circ Res (2005) 96:675-683
Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA)
NKT cell subsets in infection and inflammation  Chan WL, et al. Immun Lett (2003) 85:159-163
Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis
Regulation of ST2L expression on T helper (Th) type 2 cells  Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985
Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells
Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes  Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention.

 

How To Use

How To Use: 

This antibody allows the identification and purification of murine T helper 2 cells and all forms of murine mast cells.

 

Applications

 

  • Flow cytometry
  • immunoprecipitation