T1/ST2 (IL-33 R) Monoclonal Antibody, Biotinylated

Catalog Number: 
101001B
Qty/Size: 
0.5 mL

Monoclonal Antibody to mouse T1/ST2 (IL-33 R), clone DJ8 from MD Bioproducts

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Mouse T1/ST2 (IL-33 R) biotinylated monoclonal antibody (clone DJ8) for the identification and purification of Th2 cells and all forms of murine mast cells. 

Overview

Overview: 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.

Data/Specifications

Data/Specifications: 

Rat anti-Mouse T1/ST2 biotinylated mAb

Clone: DJ8

 

Ig Subclass: IgG1 (light chain not isotyped)

 

Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.

 

Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine-stimulated murine fibroblasts.

 

Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.

Literature/Support

Literature/Support: 

T1/ST2 Monoclonal Antibody, Biotinylated (PDF, product insert)

 

Understanding The Four Isoforms of ST2 (blog post)

 

Cui, Y., Dahlin, J. S., Feinstein, R., Bankova, L. G., Xing, W., Shin, K., ... & Hallgren, J. (2014). Mouse mast cell protease-6 and MHC are involved in the development of experimental asthma. The Journal of Immunology, 193(10), 4783-4789.

 

Kim, B. S., Wang, K., Siracusa, M. C., Saenz, S. A., Brestoff, J. R., Monticelli, L. A., ... & Artis, D. (2014). Basophils promote innate lymphoid cell responses in inflamed skin. The Journal of Immunology, 193(7), 3717-3725

 

Kato, Y., Akasaki, S., Muto-Haenuki, Y., Fujieda, S., Matsushita, K., & Yoshimoto, T. (2014). Nasal sensitization with ragweed pollen induces local-allergic-rhinitis-like symptoms in mice.

 

Komai‐Koma, M., Li, D., Wang, E., Vaughan, D., & Xu, D. (2014). Anti‐Toll‐like receptor 2 and 4 antibodies suppress inflammatory response in mice. Immunology, 143(3), 354-362.

 

Heger, K., Seidler, B., Vahl, J. C., Schwartz, C., Kober, M., Klein, S., ... & Schmidt‐Supprian, M. (2014). CreERT2 expression from within the c‐Kit gene locus allows efficient inducible gene targeting in and ablation of mast cells. European journal of immunology, 44(1), 296-306.

 

Spooner, C. J., Lesch, J., Yan, D., Khan, A. A., Abbas, A., Ramirez-Carrozzi, V., ... & Singh, H. (2013). Specification of type 2 innate lymphocytes by the transcriptional determinant Gfi1. Nature immunology, 14(12), 1229-1236.

 

Nussbaum, J. C., Van Dyken, S. J., von Moltke, J., Cheng, L. E., Mohapatra, A., Molofsky, A. B., ... & Locksley, R. M. (2013). Type 2 innate lymphoid cells control eosinophil homeostasis. Nature, 502(7470), 245-248.

 

Saenz, S. A., Siracusa, M. C., Monticelli, L. A., Ziegler, C. G., Kim, B. S., Brestoff, J. R., ... & Artis, D. (2013). IL-25 simultaneously elicits distinct populations of innate lymphoid cells and multipotent progenitor type 2 (MPPtype2) cells. The Journal of experimental medicine, 210(9), 1823-1837.

 

Kamijo, S., Takeda, H., Tokura, T., Suzuki, M., Inui, K., Hara, M., ... & Takai, T. (2013). IL-33–Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen–Induced Allergic Airway Inflammation. The Journal of Immunology, 190(9), 4489-4499.

 

Molofsky, A. B., Nussbaum, J. C., Liang, H. E., Van Dyken, S. J., Cheng, L. E., Mohapatra, A., ... & Locksley, R. M. (2013). Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages. The Journal of experimental medicine, 210(3), 535-549.

 

Nussbaum, J. C., Van Dyken, S. J., von Moltke, J., Cheng, L. E., Mohapatra, A., Molofsky, A. B., ... & Locksley, R. M. (2013). Type 2 innate lymphoid cells control eosinophil homeostasis. Nature, 502(7470), 245-248.

 

Wong, S. H., Walker, J. A., Jolin, H. E., Drynan, L. F., Hams, E., Camelo, A., ... & McKenzie, A. N. (2012). Transcription factor ROR [alpha] is critical for nuocyte development. Nature immunology, 13(3), 229-236.

 

Chang, Y. J., Kim, H. Y., Albacker, L. A., Baumgarth, N., McKenzie, A. N., Smith, D. E., ... & Umetsu, D. T. (2011). Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity. Nature immunology, 12(7), 631-638.

 

Saenz, S. A., Siracusa, M. C., Perrigoue, J. G., Spencer, S. P., Urban Jr, J. F., Tocker, J. E., ... & Artis, D. (2010). IL25 elicits a multipotent progenitor cell population that promotes TH2 cytokine responses. Nature, 464(7293), 1362-1366.

 

References/Citations:

How the T1/ST2 Biotinylated antibody was used:
Cutting Edge: Atopy Promotes Th2 Responses to Alloantigens and Increases the Incidence and Tempo of Corneal Allograft Rejection
Clay Beauregard et al., J. Immunol., Jun 2005; 174: 6577 - 6581.

Immunohistochemistry analysis to detect the presence of Th2 cells in four-micrometer cross sections of the eye. Eyes were obtained from C57/BL/6 and BALB/c mice.

Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells?  Chan WL, et al. Cellular Immun (2005) 233:109-114

Application of ST2L and IL18R markers in absdominal aortic aneurysms
Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells  Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. 
Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies.
Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells  Chan, W.L. et al., Circ Res (2005) 96:675-683
Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA)
NKT cell subsets in infection and inflammation  Chan WL, et al. Immun Lett (2003) 85:159-163
Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis
Regulation of ST2L expression on T helper (Th) type 2 cells  Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985
Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells
Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes  Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention.

 

How To Use

How To Use: 

This antibody allows the identification and purification of murine T helper 2 cells and all forms of murine mast cells.

 

Applications

 

  • Flow cytometry
  • immunoprecipitation