Mouse IgG1 Isotype Control, Purified Antibody
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Mouse IgG1 Isotype control, purified
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype:
Mouse IgG1k
Specifcity:
T-2 mycotoxin
Form:
This product supplied as 1.0 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2.
Storage:
Store at 2 – 8 °C
Literature/Support
How To Use
Applications:
- Used for Flow cytometry: working dilution of ≤1 ug/106 cells
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA): working dilution of 1 – 5 ug/mL
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA).
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs