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Adiponectin ELISA, human

Adiponectin ELISA from MD Bioproducts
Catalog Number: 
M046026
Qty/Size: 
96 wells

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For the quantitative determination of Adiponectin in human serum, plasma, urine and cell culture supernate samples

Overview

Overview: 

Adiponectin, also known as Adipocyte Complement-Related Protein of 30 kDa (ACRP30), is a secreted serum protein expressed exclusively in differentiated adipocytes. Studies indicated that decreased plasma adiponectin concentration is associated with obesity, insulin resistance essential hypertension, inflammation and atherosclerosis, and acute myocardial infarction. On the other hand, increased adiponectin level leads to nephrotic syndrome.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  urine, serum, plasma, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0 - 200 ng/mL

Sensitivity:  0.5 ng/mL

Assay Length:   2 hours 40 min

Literature/Support

Literature/Support: 

How It Works

How It Works: 

The Human Adiponectin ELISA Kit is designed for detection of adiponectin in human urine, plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures adiponectin in less than 4 hours. A polyclonal antibody specific for adiponectin has been pre- coated onto a microplate. Adiponectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for adiponectin, which is recognized by a streptavidin-peroxi - dase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

principle of the Adiponectin ELISA from MD Bioproducts

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