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Alpha-1-Acid Glycoprotein ELISA, human

human Alpha-1-Acid Glycoprotein ELISA assay For the quantitative determination o
Catalog Number: 
M046033
Qty/Size: 
96 wells

human Alpha-1 Acid Glycoprotein ELISA from MD Bioproducts

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For the quantitative determination of Alpha-1-Acid Glycoprotein in human plasma, serum and urine samples

Overview

Overview: 

Alpha-1-Acid Glycoprotein (AGP) is an acute-phase protein secreted by the liver which under conditions of inflammation increase several-fold in concentration. An elevated serum level of acute-phase inflammatory markers is associated with an increased risk of cardiovascular disease. Urinary orosomucoid excretion rate predicts cardiovascular mortality in patients with Type II diabetes. AGP can be used as a marker for inflammation, chronic alcohol drinking, chronic kidney disease and asthma.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, urine

Sample Size: 25 uL

Standard Curve Range:  0 - 4 ug/mL

Sensitivity:  0.08 ug/mL

Assay Length:   2 hours 40 min

Literature/Support

Literature/Support: 

How It Works

How It Works: 

The Human AGP ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive enzyme immunoassay technique that measures human plasma and serum, AGP in less than 3 hours. A poly - clonal antibody specific for human AGP has been pre-coated onto a 96-well microplate with removable strips. AGP in standards and samples is competed by a biotinylated AGP sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

principle of competitive assay - human alpha-1-acid glycoprotein assay

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