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Alpha-2-HS Glycoprotein ELISA, human

human Alpha-2-HS Glycoprotein ELISA For the quantitative determination of Alpha-
Catalog Number: 
96 wells

human Alpha-2-HS glycoprotein ELISA from MD Bioproducts

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For the quantitative determination of Alpha-2-HS Glycoprotein in human plasma, serum, urine and cell culture supernate samples



The alpha-2-Heremans-Schmid Glycoprotein (AHSG), also known as alpha-2-HS-Glycoprotein, or fetuin-A, is a highly glycosylated plasma protein synthesized in liver and enriched in bone. AHSG is an abundant serum protein with powerful calcification inhibitory properties. AHSG deficiency was recently linked to cardiovascular mortality in dialysis patients. While increased fetuin-A levels positively correlated with vascular calcification in patients with diabetes and mild to moderate renal impairment, an inverse relationship was observed in dialysis patients. Both chronic inflammation and uremia may contribute to exhausting fetuin-A release in the late stages of kidney disease. It has been recently reported AHSG to be decreased in the cerebrospinal fluid of patients with Alzheimer’s disease.



Species: human

Sample Type:  serum, plasma, urine, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0 - 400 ng/mL

Sensitivity:  5.0 ng/mL

Assay Length:   3 hours 40 min



How It Works

How It Works: 

Human Alpha-2-HS-Glycoprotein ELISA kit is designed for detection of human alpha-2-HS-Glycoprotein in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme im - munoassay technique that measures alpha-2-HS-Glycoprotein in 3.5 hours. A polyclonal antibody specific for alpha-2-HS-Glycoprotein has been pre-coated onto a microplate. Alpha-2-HS-Glycoprotein in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for alpha-2-HS-Glycoprotein, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


human alpha-2-HS glycoprotein ELISA