Calcitonin ELISA

calcitonin ELISA to measure calcitonin levels in human samples.
Catalog Number: 
M046010
Qty/Size: 
96 wells

calcitonin ELISA from MD Bioproducts

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Calcitonin ELISA kit to measure calcitonin levels in human samples.

Overview

Overview: 

Calcitonin, a 32-amino-acid polypeptide, is secreted primarily by the thyroidal parafollicular C-cells. Its main biological effect is to inhibit osteoclastic bone resorption as well as playing a role in calcium and phosphorus metabolism in the bone and kidney. Calcitonin supresses resorption of bone by inhibiting the activity of osteoclasts releasing calcium and phosphorus into blood. In the kidney, calcium and phosphorus are prevented from being lost in urine by reabsorption in the kidney tubules. Calcitonin inhibits tubular reabsorption of these two ions, leading to increased rates of their loss in urine.   

 

Related Terms/Symbols:
 - CALCA gene
 - CALCA1 gene
 - P01258
 - CALC_HUMAN

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:   serum

Sample Size: 100 uL

Standard Curve Range:  10 - 1000 pg/mL

Sensitivity:  1 pg/mL

Assay Length:   4.5 hrs

Literature/Support

Literature/Support: 

Product Insert:

Calcitonin ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

References/Citations:How the Calcitonin ELISA kit was used:
The RET Kinase Inhibitor NVP-AST487 Blocks Growth and Calcitonin Gene Expression through Distinct Mechanisms in Medullary Thyroid Cancer Cells
Nagako Akeno-Stuart et al., Cancer Res., Jul 2007; 67: 6956 - 6964.
Measure the concentration of calcitonin in serum of athymic mice with human medullary carcinoma (cell line TT) xenografts and in TT cell-conditioned media.


How It Works

How It Works: 

The Calcitonin Immunoassay is a two-site ELISA (Enzyme-Linked Immunosorbent Assay) for the measure- ment of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for well-defined regions on the calcitonin molecule. One antibody binds only to Calcitonin 11-23 and this antibody is biotinylated. The other antibody binds only to Calcitonin 21-32 and this antibody is labeled with horseradish peroxidase [HRP] for detection.

 

In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. Thus the calcitonin in the sample is “sandwiched” between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and patient samples are determined directly from this curve.

Calcitonin ELISA

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