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Ceruloplasmin ELISA - human

ceruloplasmin ELISA to measure Ceruloplasmin levels in human serum and plasma.
Catalog Number: 
M046029
Qty/Size: 
96 wells

ceruloplasmin ELISA from MD Bioproducts

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Ceruloplasmin ELISA kit to measure Ceruloplasmin levels in human serum and plasma.

Overview

Overview: 

Ceruloplasmin is an abundant α2-serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species. Ceruloplasmin is a copper-binding protein that normally removes iron from cells by its ferroxidase activity. Ceruloplasmin concentration on average is 14.6 (±4.0) mg/dL. Low levels of ceruloplasmin lead to the abnormal deposition of iron in cells, including those of the pancreas, liver, retina and the basal ganglia region of the brain. Some diseases associated with ceruloplasmin are Wilson’s disease. Hemochromatosis, Menkes disease and Aceroluplasminemia.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum and plasma (suggested dilution)

Sample Size: 25 uL

Standard Curve Range:  0 - 40 ug/mL

Sensitivity:  0.06 ug/mL

Assay Length:   2 hours 40 min

Literature/Support

Literature/Support: 

Product Insert:

Human Ceruloplasmin ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

The Human Ceruloplasmin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human ceruloplasmin in plasma, and serum. This assay employs a quantitative competitive enzyme immu - noassay technique that measures human ceruloplasmin in less than 3 hours. A polyclonal antibody specific for human ceruloplasmin has been pre-coated onto a 96-well microplate with removable strips. Ceruloplasmin in standards and samples is competed by a biotinylated ceruloplasmin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

human ceruloplasmin ELISA

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